CRH刺激大鼠下丘脑脑片CRH及CRH1R的表达研究

目的　通过CRH刺激大鼠下丘脑脑片 ,探讨下丘脑CRH和CRH1R在下丘脑中的分布和表达规律。方法　采用大鼠下丘脑脑片实验模型 ,运用免疫组织化学技术 ,观察CRH刺激大鼠下丘脑脑片后CRH和CRH1R在下丘脑中的分布和表达规律。结果　CRH刺激下丘脑脑片后下丘脑CRH和CRHlR含量明显增多 ,而CP 15 45 2 6、H89可显著抑制CRH和CRHlR的增加。结论　CRH刺激下丘脑脑片后增多的CRH与CRHlR结合后进一步引起下丘脑神经元CRH和CRHlR表达增加。提示在严重创伤应激反应中 ,CRH通过PKA途径实现对下丘脑神经元CRH的表达的超短正反馈调节。

Expression of corticotropin-releasing hormone and corticotropin-releasing hormone type 1 receptor in rat hypothalamic slice after stimulation with corticotropin- releasing hormone stimulation in vitro

Objective To investigate the distribution and expressions of corticotropin-releasing hormone (CRH) and corticotropin-releasing hormone type 1 receptor (CRH1R) in rat hypothalamus after CRH stimulation of hypothalamic slices in rats in vitro. Methods Model of rat hypothalamic slices was employed in this study. The distribution and expressions of corticotropin-releasing hormone and corticotropin-releasing hormone type 1 receptor in rats hypothalamus after CRH stimulation of the hypothalamic slices in rats in vitro were observed by immunocytochemical method. Results An increased intracellular concentration of CRH and CRH1R in the hypothalamus was observed after CRH stimulation of the hypothalamic slices in rats. CP-154526 or H89 significantly inhibited the expressions of CRH and CRH1R. Conclusion An increased concentration of CRH conjugated with CRH1R can induce more expressions of CRH and CRH1R in rats hypothalamus after CRH stimulation of the hypothalamic slices in rats in vitro, suggesting that the expression of CRH in hypothalamic neuron may have regulatory effect on the ultrashort positive feedback through PKA pathway.