BRD7基因转染对鼻咽癌细胞生长的抑制作用

目的：探讨鼻咽癌负相关基因BRD7对鼻咽癌细胞系HNE1生长的影响。方法：构建BRD7基因真核表达载体pcDNA3.1( )/BRD7重组体，采用脂质体介导转染技术，将BRD7真核表达重组粒和空载体质粒分别导入鼻咽癌细胞系HNE1，Southern杂交和RT－PCR分别检测外源性DNA的整合和BRD7基因的表达，并借助细胞生长曲线、软琼脂集落形成试验、流式细胞计数和裸鼠接种方法对转染细胞的生物学行为进行了检测。结果：转染BRD7基因的HNE1生长倍增时间为53h，较HNE1（23．9h)和空载体转染HNE1（24．1h）明显延长，流式细胞仪表明，BRD7表达升高延缓细胞由G0－G1期进入S期，BRD7转染HNE1在软琼脂中集落形成率较对照组显著下降（P＜0．01），裸鼠接种试验显示BRD7基因转染细胞HNE1生长速度受到抑制。结论：BRD7基因重表达有助于HNE1的恶性表型的逆转；BRD7是一个鼻咽癌相关的抑瘤基因良好的候选者。

Growth Suppression of Nasopharyngeal Carcinoma Cell through Transfection of BRD7 Gene

Objective: This study was designed to explore the effect of BRD7 gene negative-associated with nasopharyngeal carcinoma (NPC) on the growth of NPC cell line HNEI. Methods: The mammal expression vector of BRD7, pcDNA3. 1 (+ ) /BRD7, was constructed and transfected into HNE1 cell. Stable G418-resistant clones were isolated, and the integration of the exogenous vector DNA and the expression of BRD7 gene were detected by Southern blot and HT-PCR respectively. Finally the cytobiological characterization of positive clone (B-4) was analyzed by using population double time (PDT), soft agar assay, cytometry, and xenograft. Results: The PDT of G418-resistant HNE1 cell with epression of BRD7 was 53 h and significantly longer than that of vector-transfected HNE1 cell and untransfected HNE1 (P < 0. 01 ). Flow cytometric data shown that more BRD7 transfected cells went into phase G0 - G1 than controls. And it also presented decreased clonogenicity and tomorigenicity in soft agar assay and tumor formation in nude mice. Conclusion: The reexpression of BRD7 could favor the malignant phenotype revision of NPC cells. And BRD7 gene might be a good candidate of tumor suppressor gene correlated with NPC.