视黄醇结合蛋白4与糖尿病大血管病变的关系及吡格列酮的干预作用

目的研究视黄醇结合蛋白4(RBP4)、单核细胞趋化蛋白-1(MCP-1)在糖尿病动脉粥样硬化大鼠血清中的表达及吡格列酮的干预作用。方法 75只Wistar大鼠随机选取60只以高脂、高糖及链脲佐菌素诱导制成糖尿病模型,造模成功56只大鼠随机分为糖尿病组(38只)和药物干预组(干预组,18只,吡格列酮20 mg·kg~(-1)·d~(-1)灌胃)。另15只大鼠以普通饲料喂养作为对照组。3组大鼠16周后处死,取主动脉全段行HE染色,根据动脉粥样硬化的严重程度,将糖尿病组分为单纯糖尿病组(单纯组,14只)和糖尿病合并动脉粥样硬化组(合并组,24只),测空腹血糖(FBG)、空腹胰岛素(FINS)、TC、TG、HDL-C、LDL-C及血清RBP4,MCP-1,计算动脉粥样硬化指数(AIP)及胰岛素抵抗指数(HOMA-IR)。结果糖尿病组TG、LDL-C、FBG、FINS、RBP4、MCP-1、HOMA-IR、AIP高于对照组(P<0.05),干预组除RBP4与单纯组无差异外,上述指标均较单纯组和合并组下降;RBP4与TG、LDL-C、HOMA-IR、AIP、MCP-1呈正相关,与HDL-C呈负相关。RBP4、TG是糖尿病发生大血管病变的独立危险因素。结论 RBP4是糖尿病发生大血管病变的独立危险因素;吡格列酮对糖尿病大血管病变有保护作用。

Relation between RBP4 and pathological change of large vessel in diabetic rats and intervention of pioglitazone

Objective To investigate the expression of retinol binding protein4(RBP4) and monocyte chemoattractant protein-1 (MCP-1) in diabetic rats with atherosclerosis and the intervention of pioglitazone(Pio). Methods Wistar rats were randomly divided into three groups: control group(group NC, 15), streptozotocin-induced diabetic rats (group DM, 38), Pio treated diabetic rats(group intervention,18). All rats were sacrificed after 16 weeks. The rats of group DM were divided into diabetes mellitus group(group DM1) and diabetes mellitus with atherosclerosis group (group DM2) by the degree of aorta pathological changes. Blood was taken for measuring the levels of serum RBP4, serum MCP-1, glucose, insulin, HDL-C, LDL-C, TG, TC. Atherogenic index of plasma(AIP) and insulin resistance index(HOMA-IR)were calculated. Results Compared with group NC, TG, LDL-C, FBG, FINS, RBP4, MCP-1, HOMA-IR and AlP were significantly increased while HDL-C decreased in group DM. Compared with group DM1 ,above-mentioned indexes were significantly increased in group DM2, while decreased in group DM + Pio. There were positive correlation between RBP4 and TG, LDL-C, HOMA-IR, AIP, MCP-1, and negative correlation Between RBP4 and HDL-C. RBP4 and TG were independent risk factors for the progression of macrovascular complication of diabetes mellitus. Conclusion RBP4 is an independent risk factor for the progression of macrovascular complication of diabetes mellitus. Pio may have protective effect against the progression of macrovascular complication of diabetes mellitus.