北五味子总木脂素对脑缺血模型大鼠神经细胞凋亡及p-AKT表达的影响

目的:观察北五味子总木脂素(Schisandra chinensis lignans,SCL)对局灶性脑缺血损伤大鼠神经细胞凋亡和PI3K/AKT信号传导途径的影响,并探讨其作用机制。方法:用北五味子总木脂素高、中、低剂量组(100,50,25 mg·kg-1)给大鼠分别灌胃给药,连续14 d后,线栓法建立脑缺血损伤模型,进行神经功能评分,TTC染色观察大鼠的脑梗死面积,HE染色观察脑组织的病理形态学改变,免疫组化检测脑组织中Bcl-2和Bax的表达,Western blotting检测脑组织内p-AKT和AKT蛋白的表达情况。结果:与模型组比较,北五味子总木脂素高、中、低剂量组均能不同程度缩小脑梗死面积;改善脑组织的病理形态学改变;促进抗凋亡蛋白Bcl-2的表达,抑制促凋亡蛋白Bax表达,同时促进p-AKT的表达。结论:北五味子总木脂素对大鼠脑缺血性损伤具有一定的保护作用,机制可能与其促进p-AKT活性增加,提高脑组织抗缺血损伤的能力和抑制神经细胞的凋亡有关。

Effect of Schisandra chinensis lignans on neuronal apoptosis and p-AKT expression of rats in cerebral ischemia injury model

Objective: To observe the effect of Schisandra chinensis lignans(SCL) on neuronal apoptosis and PI3K/AKT signaling pathway of rats in the cerebral ischemia injury model, and study its possible mechanism.Method: Rats were orally administered SCL high, middle and low dose groups (100, 50, 25 mg·kg^-1) for 14 days.The cerebral ischemia injury model was established by using the suture-occluded method to rate the neurological functions.The cerebral infarction area was observed by TTC staining.The pathological changes in brain tissues were determined by HE staining.Bcl-2 and Bax expressions were detected by immunohistochemical assay.The protein expressions of p-AKT and AKT were assayed by Western blotting.Result: Compared with the model group, SCL high, middle and low dose groups showed reduction in the cerebral infarction area to varying degrees, improve the pathological changes in brain tissues, promote the expression of apoptin Bcl-2 and p-AKT, and inhibit the expression of apoptin Bax.Conclusion: SCL shows a protective effect on rats with cerebral ischemia injury.Its mechanism may be related to the increase in p-AKT ability and anti-ischemic brain injury capacity and the inhibition of nerve cells.