FISH to mitotic chromosomes and extended DNA fibres of Beta procumbens in a series of monosomic additions to beet (B. vulgaris) |
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Authors: | Mahmoud Mesbah Jannie Wennekes-Van Eden J Hans De Jong Theo S M De Bock Wouter Lange |
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Institution: | (1) Plant Research International, Wageningen University and Research Centre, PO Box 16, NL-6700 AA Wageningen, The Netherlands;(2) Laboratory of Genetics, Wageningen University and Research Centre, Dreijenlaan 2, NL-6703 HA Wageningen, The Netherlands |
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Abstract: | The physical localization and organization of a Procumbentes-specific repetitive DNA sequence, PB6-4, on the chromosomes of Beta procumbens (2n=18) were studied, using FISH (fluorescence in situ hybridization) to mitotic chromosomes and extended DNA fibres. The chromosomes of B. procumbens were studied in metaphase complements of the species itself, as well as in preparations of a series of eight different B. procumbens-derived monosomic additions to B. vulgaris (2n=18). FISH to chromosome spreads of B. procumbens revealed that PB6-4 hybridizes to all chromosomes, predominantly in the pericentromeric regions, but with differences in size and brightness
of the signals. Hybridization of PB6-4 to metaphase complements of B. vulgaris revealed no signals, indicating that cross-hybridization with the genome of this species was negligible. Consequently, hybridization
of PB6-4 to metaphase complements of the monosomic additions yielded fluorescent signals on the alien chromosomes only. The previously
observed differences in size and brightness of the fluorescent spots were confirmed using the single alien chromosomes. FISH
of PB6-4 to extended DNA fibres of the monosomic additions indicated differences in the fluorescent track lengths between the alien
chromosomes. Measurements of the fluorescent tracts allowed classification into discrete groups, varying from one to three
groups per B. procumbens chromosome. The data revealed that the brightness or size of the signal at mitotic metaphase and the length of the fluorescent
tracks on the DNA fibres were correlated.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Beta procumbens Beta vulgaris extended DNA fibres fluorescence in-situ hybridization (FISH) monosomic additions |
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