鼻咽癌细胞bcl—2基因异常与EB病毒感染的关系

目的 研究鼻咽癌细胞bcl－2基因异常与EB病毒感染的关系。方法 采用半套式原位聚合酶链反应和免疫组织化学技术对41例鼻咽癌细胞中bcl－2基因重排、bcl－2蛋白表达率为82．92％（34／41）、LMP－1的阳必率为51．2％（21／41），bcl－2基因异常（重排和蛋白异常表达）与LMP－1的阳性率无显著性差异（P＜0．05），bcl－2／JH融合基因形成与bcl－2蛋白表达无显著性差异（P＜0．05）。结论 bcl－2基因在鼻咽癌发生过程中可能起一定作用。mbr和mcr区域同为bcl－2基因在鼻咽癌的2个重要断裂区；bcl－2基因重排可能不是引起bcl－2蛋白异常表达惟一原因；EBV感染对bcl－2基因重排和bcl－2蛋白过表达可能无影响。

bcl-2 Gene Aberrantion in Nasopharyngeal Carcinoma and its Relation with EBV Infection

Objective To study the relationship between bcl 2 gene aberrantion and EBV infection in nasopharyngeal carcinoma(NPC).Methods bcl 2/JH fusion gene,bcl 2 protein and latent membrane protein 1(LMP 1) in 41 cases of NPC were detected by semi nested in situ PCR and immunohistochemistry technique.Results 14.6%(6/41) of NPC were found to have bcl 2 gene rearrangement.34 cases of NPC expressed bcl 2 protein,the positive incidence was 82.9%.The positive rate of LMP 1 was 51.2%(21/41).There was no correlation between EBV infection and bcl 2 gene aberrant(gene rearrangement and protein overexpression).Conclusion These results suggest that bcl 2 gene might play an role in the pathogenesis of NPC.Mbr and mcrare should be the two important breakpoints of bcl 2 gene in NPC;Bcl 2 gene rearrangement may not the only cause leading to bcl 2 protein overexpression.The results also indicate that EBV infection might not influence on bcl 2 gene rearrangement and bcl 2 protein overexpression.