利用图像分析技术对基因转导至颌下腺后腺体组织的研究

目的通过图像分析仪研究腺病毒介导荧光素酶基因转导至大白鼠颌下腺后腺体组织结构变化。方法将40只Wistar大鼠随机分为5组,经颌下腺导管转导腺病毒荧光素酶基因重组体即AdCMVLuc,3d,1、2、4、8周后观察其基因表达及病理变化。结果颌下腺转基因表达3d最高,以后逐渐下降,至4、8周时仍可测到表达。组织结构:3d~1周可见腺细胞核面积和周长增大,2周腺细胞核面积和周长开始减小,8周腺细胞核面积和周长基本恢复正常。结论腺病毒介导的大鼠颌下腺基因转导后腺体有明显炎症反应,腺细胞核面积和周长有明显的变化,提示腺体蛋白合成体系功能活跃。

CMIAS-assisted histological study of submandibular gland in rats after adenovirus-mediated gene transferring

Objective To investigate histological changes of submandibular glands (SMG) in rats after adenovirus-mediated gene transferring using CMIAS. Methods Forty Wistar rats were randomized into five groups to receive adenovirus-mediated luciferase gene (AdCMVLuc, 108pfu) transferring into the submandibular glands and observed for histological changes of SMG after 3 days and at 1, 2, 4 and 8 weeks. Results Peak expression was observed in animal models three days following administration of the vector. Expression of the transferred gene in submandibular glands declined gradually, but remained detectable at 4 and 8 weeks. Histologically, the area and circumference of SMG cellular nuclei increased from day 3 to week 1, and gradually decreased starting from week two, until resuming normal size in week 8. Conclusion Adenovirus-mediated gene transfer induces remarkable inflammatory changes in rat SMG. Obvious pathological changes in the nuclei suggested high protein synthesis activities in the acinar cells after gene transfer.