喉癌组织中P53和COX-2及VEGF与微血管密度相关性研究

目的探讨喉癌组织中P53、环氧合酶-2(cyclooxygenase-2,COX-2)及血管内皮生长因子(vascular endothelial growth factor,VEGF)与微血管密度(microvessel density,MVD)的关系。方法通过免疫组化S-P法对64例喉癌组织标本中P53、COX-2、VEGF及CD34抗体标记的MVD进行检测。结果喉癌组织中P53、COX-2、VEGF的阳性表达率分别为62.50%(40/64)、68.75%(44/64)和71.88%(4 6/6 4),MVD为3 5.8 7±9.3 8。P5 3、COX-2与VEGF之间呈正相关(r=0.3 8 3,P=0.0 0 2;r=0.305,P=0.014)。在有淋巴结转移的肿瘤中P53、COX-2及VEGF阳性表达率及MVD明显高于非转移组(P<0.05)。结论 P53、COX-2及VEGF表达可能共同参予喉癌的血管形成,且COX-2、VEGF的表达可能由P53调节,共同参与颈淋巴结转移的过程。

The correlations among the expression of P53,COX-2,VEGF and microvessel density in laryngeal carcinoma

Objective To evaluate the correlations among the expression of P53,COX-2,VEGF and microvessel density(MVD) in laryngeal carcinoma.Methods Immunohistochemical staining was applied to detecting the expression of P53,COX-2,VEGF proteins and MVD in 64 surgically resected specimens of laryngeal carcinoma.Results Positive expression rates of P53,COX-2,and VEGF proteins were 62.50%,68.75%,and 71.88% respectively.The positive expression rates of P53,COX-2 and VEGF proteins and the MVD values in specimens with cervical lymphatic metastasis were significantly higher than those without metastasis(P<0.05).The mean MVD was 35.87±9.38.The expression of P53 and COX-2 was positively correlated with that of VEGF(r=0.383,P=0.002;r=0.305,P=0.014).Conclusion P53,COX-2 and VEGF proteins may play an important roles in the angiogenesis of laryngeal carcinoma.Furthermore,the expression of VEGF and COX-2 may be regulated by P53,involving in the process of cervical lymphatic metastasis of laryngeal carcinoma cells.