| 利用噬菌体肽库筛选HeLa细胞表面与人CD59结合的活性短肽 |
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| 引用本文： | 崔林林,高美华,王冰.利用噬菌体肽库筛选HeLa细胞表面与人CD59结合的活性短肽[J].中国免疫学杂志,2007,23(4):349-351. |
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| 作者姓名： | 崔林林 高美华 王冰 |
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| 作者单位： | 青岛大学医学院免疫教研室,青岛,266021 |
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| 摘 要： | 目的寻找HeLa细胞表面与人CD59特异性结合的短肽序列。方法分别用非转基因HeLa细胞和转染CD59基因的HeLa细胞的裂解蛋白进行5轮亲和筛选,并通过竞争结合实验,然后用ELISA方法筛选噬菌体阳性克隆。提取单克隆DNA测序,并对其进行DNA序列分析推导出短肽序列。结果随机挑选的16个单克隆中有10个克隆对HeLa细胞有特异性结合力,经测序得到一条高度同源的多肽序列。结论通过噬菌体随机肽库对肿瘤细胞进行全细胞蛋白筛选,得到了噬菌体多肽能高特异性与肿瘤细胞结合的短肽序列,为下一步设计肿瘤逃逸相关的CD59活性位点的短肽药物提供了参考依据。
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| 关 键 词： | HeLa细胞 噬菌体肽库 人CD59蛋白 肿瘤逃逸 |
| 文章编号： | 1000-484X（2007）04-0349-03 |
| 修稿时间： | 2006-06-052006-09-11 |
Specific coalescent peptides binding with CD59 on HeLa cells screened by phage peptide library |
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| CUI Lin-Lin,GAO Mei-Hua,WANG Bing.Specific coalescent peptides binding with CD59 on HeLa cells screened by phage peptide library[J].Chinese Journal of Immunology,2007,23(4):349-351. |
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| Authors: | CUI Lin-Lin GAO Mei-Hua WANG Bing |
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| Institution: | Department of Immunology,Medical College of Qingdao University, Qingdao 266021, China |
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| Abstract: | Objective:To search for carrier of short active peptide with binding to human CD59 of the surface of HeLa cell.Methods:Ph. D. 12 display peptide library was biopanning with HeLa cell high-expressing CD59. After competitive test, positive phage clones were identified by sandwich ELISA and sequenced.Results:10 phage clones were identified to have higher combination with CD59.Conclusion:The sequence of peptide with binding to CD59 has been obtained.That might lay a basis for design of short peptide of CD59 on tumor sneaking through. |
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| Keywords: | HeLa cell Peptide library Human CD59 protein Tumor escape |
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