活体溶出伏安法测定鼠脑缺血时纹状体单胺神经递质的含量变化

应用活体溶出伏安法（ＩＶＶ）连续测定沙土鼠脑缺血再灌流模型纹状体单胺类神经递质多巴胺（ＤＡ）５－羟吲哚乙酸（５－ＨＩＡＡ），并同期测定鼠脑皮质灌流量、脑电活动及腐胶含量。结果发现：１、脑缺血期纹状体ＤＡ与５－ＨＩＡＡ显著增高；再灌流期ＤＡ明显下降，５－ＨＩＡＡ仅在１５ｍｉｎ时有一过性降低，但两者均明显高于缺血前水平。２、脑缺血时脑皮质灌流量显著下降（下降９５％），相应时间的脑电活动明显抑制；再灌流期皮质灌流量有所恢复，但仍明显低于缺血前（约为其３０％），脑电活动亦无恢复。３、脑缺血时脑皮质腐胺含量下降，而再灌流３０ｍｉｎ时显著增高，在９０ｍｉｎ时恢复至正常水平。

The changes in striatal monoamine neurotransmitter measured by IVV during brain ischemia in gerbils

The changes on dopamine (DA) and 5 -hydroxyindoleacetic acid (5 -HIAA)were determined con tinuously in the striatum using in vivo voltammetry (IVV), Regional cortical blood flow, EEG, and the content of putrescine in the regional conical homegenate were also determined in the meantime. The main results were as follows: 1.The content of DA and 5-HIAA increased during 30 min of brain ischemia. Following reperfusion DA decreased rapidly but being higher than control values and 5-HIAA exceed control values during 90 min reperfusion, despite of decreas at 15 min. 2.During brain ischemia, the regional cortical blood flow decreased markedly by 95%, at the same time the activity of EEG was inhibited profoundly. The blood flow increased slightly during the reperfusion period, but still lower than control values(about 30% by control).The activity of EEG did not recover during the period of 90 min reperfusion. 3. During the ischemia period, the content of putrescine decreased but it increased dramatically at 30 min reperfusion; then it recovered to the level of control at the reperfusion of 90 min.