| 骨调素和单核细胞趋化蛋白在大鼠梗阻性肾病中的表达及可能作用 |
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| 引用本文： | 水华,王群.骨调素和单核细胞趋化蛋白在大鼠梗阻性肾病中的表达及可能作用[J].中国中西医结合肾病杂志,2006,7(5):269-271. |
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| 作者姓名： | 水华 王群 |
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| 作者单位： | 武汉大学中南医院肾内科,武汉,430071 |
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| 摘 要： | 目的:探讨骨调素(OPN)和单核细胞趋化蛋白(MCP-1)在大鼠梗阻性模型中的表达及其在肾脏纤维化发病机制中的作用.方法:采用-单侧输尿管结扎制造梗阻性肾病模型,分别于造模后7 d、14 d取肾组织,应用HE染色观察肾脏病理改变,免疫组化方法检测肾组织畔OPN和MCP-1蛋白的表达,应用逆转录-聚合酶链式反应(RT-PCR)法观察肾组织中OPN mRNA和MCP-1 mRNA的变化.结果:OPN、MCP-1表达主要位于肾小管上皮细胞,随着梗阻时间的延长,肾组织中OPN、MCP-1蛋白和mRNA表达明显增加.结论:OPN、MCP-1蛋白和mRNA在梗阻性肾病大鼠肾组织表达明显增加介导炎症过程,参与肾间质纤维化.
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| 关 键 词： | 尿道梗阻 骨调素 单核细胞趋化蛋白 肾纤维化 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2006-02-16 |
Expression and Functional Role of OPN and MCP-1 in the Kidney After Unilateral Ureteral Obstruction in Rts |
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| SHUI Hua,WANG Qun.Expression and Functional Role of OPN and MCP-1 in the Kidney After Unilateral Ureteral Obstruction in Rts[J].Chinese Journal of Integrated Traditional and Western Nephrology,2006,7(5):269-271. |
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| Authors: | SHUI Hua WANG Qun |
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| Abstract: | Objective:To investigate the expression and functional role of OPN and MCP-1 in the kidney after unilateral ureteral obstruction in rats.Methods:Unilateral ureteral obstruction(UUO) models were induced by ligation of the left ureter.Rats were sacrificed at 7 and 14 days after UUO.Renal tissues were examined by light microscopy.Immunhistochemical staining was applied to measure the expression of OPN and MCP-1.RT-PCR was performed to determine the expresstion of OPN and MCP-1 mRNA.Results:In comparison with control group,the expression of OPN,MCP1 protein and mRNA significantly increased(P<0.01).Conclusion:The protein and mRNA of OPN and MCP-1 significantly increase in the obstructed kidney,which may cause renal fibrosis. |
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| Keywords: | Ureteral obstruction Osteopontin Monocyte chemoattractant protein Renal fibrosis |
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