黄芪注射液对H22肝瘤小鼠的抑瘤作用及免疫功能的影响

目的:研究黄芪注射液对小鼠H22移植瘤的生长抑制作用及免疫功能的影响。方法:采用移植性H22肝癌小鼠模型,随机分为模型对照组,5-Fu处理组,黄芪注射液高、中、低剂量组。分别连续腹腔注射5-Fu(25 mg.kg-1),黄芪注射液(12 g.kg-1、8g.kg-1和4g.kg-1)10 d后,处死小鼠,计算抑瘤率、外周血白细胞数、脾指数、胸腺指数I、L-2、TNF-α等指标,分析黄芪注射液对小鼠H22移植瘤的生长抑制作用及免疫功能的影响。结果:与模型组相比,黄芪注射液低、中、高剂量组能显著减少移植瘤瘤重,其抑瘤率分别为18.23%,31.49%,43.09%,其中黄芪注射液高剂量组与CTX组抑瘤率比较,抑瘤率有显著性差异(P<0.05)。黄芪注射液各组可不同程度地升高荷瘤小鼠的白细胞数和胸腺指数、脾指数,且能提高荷瘤小鼠血清中IL-2和TNF-α的水平(P<0.05)。结论:黄芪注射液对小鼠H22移植瘤具有明显的抑瘤作用,其作用可能与增强机体的免疫功能有关。

Anti-tumor and immunomodulatory effects of Astragalus injection on H22 bearing mice

Objective：To investigate the anti-tumor and immunomodulatory effects of Astragalus injection on H22 bearing mice.Methods： Hepatic cancer H22 model in mice was performed.After 10 days treatment,the tumor inhibitory rate,the count of peripheral white blood cells,index of thymus and spleen were calculated.IL-2 and TNF-α were measured by RIA.Results：Astragalus injection in different dose（4,8,12 g·kg-1） inhibited the growth of H22 tumor.The tumor inhibitory rates were 18.23%,31.49%,43.09%,respectively.Compared with those in 5-Fu group,the count of WBC（P〈0.05）,the indexes of thymus and spleen（P〈0.05） and the level of IL-2 and TNF-α were significantly elevated in all Astragalus injection.Conclusion：Astragalus injection has significant anti-tumor effects in vivo,probably via the improved immune function.