白藜芦醇对糖尿病肾病大鼠醛糖还原酶的影响

目的:观察白藜芦醇对糖尿病肾病大鼠醛糖还原酶的影响。方法:将48只雄性SD大鼠随机分为空白对照组、模型组、白藜芦醇低、中、高剂量组和依帕司他组(n=8)。除正常对照组外,其余各组大鼠腹腔注射STZ(55 mg.kg-1),成功造模后白藜芦醇组分别给予5、154、5 mg.kg-1白藜芦醇,依帕司他组给予10 mg.kg-1依帕司他,连续灌胃6 w后,检测大鼠血糖、体重,HE染色检测肾脏病理改变,ELISA法检测醛糖还原酶(AR)活性。结果:与正常对照组相比,糖尿病组血糖与AR活性明显升高(P<0.05),与模型组比较,白藜芦醇各组血糖与AR活性明显降低(P<0.05)。结论:白藜芦醇可降低糖尿病大鼠血糖,其降糖效应可能与抑制AR活性有关。

Effects of resveratrol on aldose reductase in diabetic nephropathy rats

Objective：To study the effects of resveratrol on aldose reductase（AR） in diabetic nephropathy rats.Methods： 48 male SD rats were randomly divided into control group,model group,epalrestat group,resveratrol low,media,high dosegroup（n=8）.Diabetesmodel was established by streptozotocin（STZ,55 mg·kg-1,i.p.）.resveratrol（5,15,45 mg·kg-1,respectively） was administrated by oral for 6 weeks.10 mg kg-1epalrestat was administrated in epalrestat group.The blood glucose,weight were measured.The pathological changes in the kidney were measured by hematoxylin and eosin stain,aldose reductase activity in kidney were detected by ELISA.Results： Compared with normal group,AR activity and blood glucose level were increased obviously in model group.Compared with model group,AR activity and blood glucose level were decreased obviously in resveratrol groups.Conclusions： Resveratrol could decreasethe blood sugar level,whichis related with decreasing AR activity.