钢铁作业工人热应激蛋白70抗体水平的血清流行病学调查

目的：探讨血清热应激蛋白70（HSP70）抗体作为工人长期接触某些环境应激后健康监测指标的可行性。方法：采用Western blot免疫印迹检测接触不同有害作业环境因素和相对无有害接触的某钢铁企业764名职工的血清HSP70抗体水平。结果：在排除年龄，工龄以及性别的影响后，多种有害接触均可使工人血清HSP70抗体水平升高。暴露于有害环境，如高温和粉尘和联合暴露，以及粉尘（石灰）暴露和噪声暴露工人血清HSP70抗体水平均明显高于对照组（OR值为2．3563－3．1526，P＜0．01），而且噪声组工龄与HSP70抗体的产生具有相关性，且差异有显著性（OR＝2．040，P＝0．0103）。结论：血清HSP70抗体水平有可能作为评价工人经历某些环境应激（如粉尘，噪声）的血清学标记物或环境危害监测指标。

Seroepidemiological investigation on the presence of antibodies to heat shock protein 70 (HSP70) in inte grated iron and steel workers exposed to abnormal

Objective To explore whether antibodies to the main heat shock proteins 70(HSP70) in serum of workers are related to prolonged exposure to abnormal stress in their working environment and whether anti HSP70 antibodies can be useful biomarkers to assess these abnormal stress. Method Western blotting was used to investigate the presence of antibodies against HSP70 in serum of 764 workers exposed to high temperature,inorganic dust,noise or to relative normal condition(control). Results After excluding the influence of age,length of service and sex,antibodies to HSP70 were found more frequently in serum of workers who had experienced prolonged abnormal stress in their working environment,such as inorganic dust(calcarea),noise and the combined action of high temperature with coal dust ( OR from 2.356 3 to 3.152 6, P <0.01).Length of service of noise group was significantly correlated with antibodies to HSP70( OR =2.040 6, P =0.010 3). Conclusion Antibodies to the main HSP70 in serum may serve as biomarkers to assess prolonged exposure to some abnormal stress such as inorganic dust(coal and calcarea),noise.