| HPLC法测定消肿止痛巴布膏中栀子苷的含量 |
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| 引用本文： | 韦金燕,陆瑞勇.HPLC法测定消肿止痛巴布膏中栀子苷的含量[J].中国民族民间医药杂志,2012(10):39-40. |
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| 作者姓名： | 韦金燕 陆瑞勇 |
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| 作者单位： | 广西壮族自治区花红药业股份有限公司,广西柳州541004 |
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| 摘 要： | 目的：建立HPLC法测定消肿止痛巴布膏中栀子苷的含量。方法：色谱柱采用Kromasil C18柱（250mm×4．6mm，5μm）；流动相：甲醇-水（27：73），检测波长：240nm；流速：1．0ml·min^-1；柱温：30℃；结果：栀子苷进样量在0．06552～1．3104μg范围内线性关系良好（r=0．9999，n=6）；平均回收率为98．5％，RSD=1．61％（n=6）。结论：所建立的方法准确、简便、快速，适用于消肿止痛巴布膏质量控制。
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| 关 键 词： | 消肿止痛巴布膏 栀子苷 HPLC法 |
Determination of Gardenoside in Xiaozhong zhitong cataplasm by HPLC |
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| WEI jin-yan,LU rui-yong.Determination of Gardenoside in Xiaozhong zhitong cataplasm by HPLC[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2012(10):39-40. |
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| Authors: | WEI jin-yan LU rui-yong |
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| Institution: | Guangxi Zhuang Autonomous Region Huahong Pharmaceutical Industry Co. , Ltd. , Liuzhou 541004, China |
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| Abstract: | Ojective： To establish an analytical method of determinating Gardenoside in Xiaozhong Zhitong cataplasm by HPLC. Methods： Gardenoside in the cataplasm were separated by HPLC on Cls column （250mm ~ 4. 6mm, 5μm） with a methanol -water （27：73） as mobile phase and detected at 240 nm. The flow rate was 1.0ml · min-1 and the column temperature was 30℃. Results： The calibration curves showed good linear over the range of 0. 06552 ~ 1. 3104txg, （r =0. 9999, n =6 ） for Gardenoside, the average recovery rate were 98.5% with RSD 1.61% （ n =6）. Conclusion： The method is simple, accurate, rapid and can be used to control the quality of the Xiaozhong Zhitong cataplasm. |
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| Keywords: | Xiaozhong Zhitong cataplasm Gardenoside HPLC |
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