肾衰养真胶囊对慢性肾衰竭大鼠脂代谢紊乱的影响

目的 观察肾衰养真胶囊对慢性肾衰竭(CRF)大鼠脂代谢紊乱的治疗效果并探讨其可能机制。方法 对50只SD雄性大鼠采用5／6肾切除法制作CRF大鼠模型．随机分为空白模型对照组、吉非罗齐治疗组及肾衰养真胶囊高、中、低剂量治疗组；另设正常对照组。于喂药4周后，观察各组脂谱及脂蛋白谱，并用反转录聚合酶链式反应(RT-PCR)法检测心肌脂蛋白脂酶信使RNA(LPLmRNA)的表达。结果 两药均能降低总胆固醇、甘油三酯、低密度脂蛋白胆固醇，升高高密度脂蛋白胆固醇．同时LPLmRNA表达上调，肾衰养真胶囊各治疗组之间无差异。结论 肾衰养真胶囊可通过促进LPLmRNA转录而调节血脂水平，改善脂蛋白谱，这可能是肾衰养真胶囊改善CRF大鼠脂代谢紊乱的机制之一。

Effects of Shenshuai Yangzhen capsule on lipid metabolism disorder in rats with chronic renal failure]

OBJECTIVE: To investigate the therapeutic effect of Shenshuai Yangzhen capsule, a preparation of traditional Chinese medicine, on lipid metabolism disorder in rats with chronic renal failure (CRF) and explore its mechanism. METHODS: Fifty male SD rats received 5/6 nephrectomy for preparation of CRF models and were randomized into CRF group, gemfibrozil group, high-, moderate- and low-dose Shenshuai Yangzhen groups, and normal control group. After 4-week treatment as indicated, myocardial lipoprotein lipase messenger RNA (LPL mRNA) level were measured by RT-PCR in rats with surgically induced renal failure (two-stage subtotal nephrectomy). The blood lipid parameters in CRF rats were also determined. RESULTS: Compared with those in the CRF group, the plasma total cholesterol, triglyceride, low-density lipoprotein cholesterol and very-low-density lipoprotein cholesterol concentrations in the treatment groups were significantly lower with substantially elevated plasma high-density lipoprotein cholesterol and LPL gene expression. No significant differences were noted between different dose groups of Shenshuai Yangzhen. CONCLUSION: Shenshuai Yangzhen capsule can regulate blood lipid levels in rats with renal insufficiency possibly by enhancing LPL gene expression.