三七总皂甙及单体Rb1对大鼠心肌缺血再灌注的保护效应

为研究三七总皂甙（PNS）及单体Rb1预处理对大鼠心肌的保护效应及其与热休克蛋白（HSP）的关系，采用在体缺血再灌注模型，实验分空白组、对照组、PNS组、Rb1组4组，心肌缺血40min再灌注30min检查心肌的超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量，用免疫组化法测缺血40min再灌120min的HSP70的表达及蛋白激酶C（ε-PKC）的含量。结果显示，与对照组相比，PNS组和Rb1组减少MDA的产生（P＜0.01)；同时，HSP70的表达，PNS组明显高于Rb1组及对照组（P＜0.01)，PNS组的ε-PKC的含量高于对照组（P＜0.01)。结果表明三七总皂甙长时间处理可促进HSP70的表达，对心肌再灌注损伤有良好的保护效应。

Protective Effect of Pretreatment with Panax Notoginseng Saponins and Rb1 on Myocardial Ischemia and Reperfusion Injury in Rats

The purpose is to study the effect of panaxtnotoginsengsaponins(PNS) pretreatment or Rb1 on myocardial ischemia/reperfusion injury in rats and the relation among protective effect of PNS or Rb1,heat shock protein 70 and ε-PKC.Forty-two SD rats were dividrd into four group:Normal group(n=6),Control P group(n=12), PNS group(n=12) and Rb1 group(n=12).Malondialdehyde(MDA) and superoxide dismutase (SOD)contents were determined after 40 min ischemia followed 30 min reperfusion 。HSP70 and ε-PKC were detected by immunohistochemical technique after reperfusion of 120 min.The results showed that: As compared with the control,PNS snd Rb1 groups can significantly reduce MDA production and protect SOD activity(P＜0.01).MDA production in PNS group was less than that in Rb1 group(P＜0.01) and SOD activity in PNS group was less than that in Rb1 group(P＜0.05).The HSP70 levels in PNS were higher than that of the control and Rb1 grougs (P＜0.01).Compared with the normal,ε-PKC in PNS group was reduced slightly (P＞0.05).In the control and Rb1 groups ε-PKC were reduced significantly (P＜0.01).The results indicated that PNS can improve the expression of heat shock protein 70 in pretreated rats and has better protective effects than that of Rb1 against myocardial ischemia/reperfusion injury.