| 梯度洗脱高效液相色谱法同时测定大豆异黄酮中大豆苷和黄豆黄苷的含量 |
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| 引用本文： | 茹波,张月辉,张瑜. 梯度洗脱高效液相色谱法同时测定大豆异黄酮中大豆苷和黄豆黄苷的含量[J]. 实用药物与临床, 2011, 14(2): 138-140 |
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| 作者姓名： | 茹波 张月辉 张瑜 |
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| 作者单位： | 1. 辽宁省药物研究院,沈阳,110015
2. 沈阳泷浦科技有限公司,沈阳,110021 |
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| 摘 要： | 目的建立大豆异黄酮中大豆苷和黄豆黄苷的含量测定方法。方法采用高效液相色谱法,色谱柱为Phenomenex Luna C18(4.6 mm×250 mm,5μm),以乙腈、水混合体系为流动相,流速为1.0 mL/min,柱温25℃,检测波长为260 nm。结果大豆苷在49.3～147.9 mg/L浓度范围内、黄豆黄苷在40.1～120.3 mg/L浓度范围内,与峰面积呈良好的线性关系,相关系数分别为0.999 7和0.999 4。方法平均回收率分别为98.7%(RSD=0.38%)和98.9%(RSD=0.60%)。结论该方法简便、准确,重现性好,可作为大豆异黄酮质量控制方法之一。
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| 关 键 词： | 大豆异黄酮 大豆苷 黄豆黄苷 高效液相色谱法 |
Determination of daidzin and glycitin in soybean isoflavones by elution gradient HPLC |
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| RU Bo,ZHANG Yue-hui,ZHANG Yu. Determination of daidzin and glycitin in soybean isoflavones by elution gradient HPLC[J]. Practical Pharmacy and Clinical Remedies, 2011, 14(2): 138-140 |
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| Authors: | RU Bo ZHANG Yue-hui ZHANG Yu |
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| Affiliation: | RU Bo1,ZHANG Yue-hui1,ZHANG Yu2(1.Pharmaceutical Research Institute of Liaoning Province,She-nyang 110015,China,2.Shenyang Longpu Science and Technology Co.,Ltd,Shenyang 110021,China) |
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| Abstract: | Objective To develop a gradient elution HPLC method for determination of daidz in and glycitin insoybean isoflavones.Methods The HPLC was used with a Phenomenex Luna C18(4.6 mm×250 mm,5 μm) columnand a mobile phase of acetonitrile-water.The flow rate was 1.0 mL/min,and column temperature was 25 ℃.The de-tection wavelength was set at 260 nm.Results The calibration curve was linear within the range of 49.3～147.9 mg/L for Daidzin(r=0.999 7) and 40.1～120.3(r=0.999 4) for Glycitin,the average recoveries were 98.7%(RSD=0.38%) for daidzin and 98.9%(RSD=0.60%) for glycitin.Conclusion This method is convenient,accurate and re-producible for determination of daidz in and glycitin in soybean isoflavones. |
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| Keywords: | Soybean isoflavones Daidzin Glycitin HPLC |
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