| HPLC-ELSD法同时测定新氢化可的松乳膏中硫酸新霉素和氢化可的松的含量 |
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| 引用本文： | 徐兴亚,李倚云.HPLC-ELSD法同时测定新氢化可的松乳膏中硫酸新霉素和氢化可的松的含量[J].实用药物与临床,2011,14(2):140-142. |
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| 作者姓名： | 徐兴亚 李倚云 |
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| 作者单位： | 1. 江苏省扬州市疾病预防控制中心皮肤病性病防治所,扬州,225009
2. 江苏省扬州市药品检验所,扬州,225008 |
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| 摘 要： | 目的建立HPLC-蒸发光散射检测器(ELSD)法,同时测定新氢化可的松乳膏中硫酸新霉素和氢化可的松的含量。方法色谱条件:C18色谱柱(150 mm×4.6 mm,5μm);流动相:乙腈-0.1 mol/L三氟乙酸溶液(梯度洗脱);漂移管温度:110℃;载气流速:2.6 L/min。用硫酸新霉素和氢化可的松对照品分别测定新氢化可的松乳膏中硫酸新霉素和氢化可的松的含量。结果硫酸新霉素进样量在0.7～4.3μg范围内(r=0.999 7),氢化可的松进样量在1.0～2.9μg范围内(r=0.999 9)。结论该方法快速、简便、重现性好,灵敏度高。
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| 关 键 词： | 高效液相色谱法 蒸发光散射检测器 氢化可的松乳膏 硫酸新霉素 氢化可的松 |
Determination of neomycin sulfate and hydrocortisone in hydrocortisone acetate ointment by HPLC-ELSD |
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| XU Xing-ya,LI Yi-yun.Determination of neomycin sulfate and hydrocortisone in hydrocortisone acetate ointment by HPLC-ELSD[J].Practical Pharmacy and Clinical Remedies,2011,14(2):140-142. |
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| Authors: | XU Xing-ya LI Yi-yun |
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| Institution: | XU Xing-ya1,LI Yi-yun2(1.Yangzhou Prevention and Cure Institute of Dermato-Venereolgy,Yangzhou 225009,China,2.Yangzhou Institute for Drug Control,Yangzhou 225008,China) |
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| Abstract: | Objective To determine the content of neomycin sulfate and hydrocortisone in hydrocortisone acetate ointment by HPLC-ELSD. Methods C18(150 mm×4.6 mm,5 μm)column was used,mobile phase:acetonitrile-0.1 mol/L trifluoroacetic acid(gradient elution),the temperature of drift tube was set at 110 ℃,and flow rate of gas was 2.6 L/min.The contents of neomycin sulfate and hydrocortisone in hydrocortisone acetate ointment were determined. Results The calibration curve of neomycin was linear in the range of 0.7～4.3 μg(r=0.999 7).The calibration curve of hydrocortisone was linear in the range of 1.0～2.9μg(r=0.999 9). Conclusion The method is rapid,sensitive,accurate and convenient. |
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| Keywords: | HPLC Evaporative light scattering detector(ELSD) Hydrocortisone acetate ointment Neomycin sulfate Hydrocortisone |
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