一种新的将神经球分散为单细胞悬液的方法

目的比较3种将神经球打散成单细胞悬液的方法对神经干细胞活性的影响。方法将16~20周人胚胎神经干细胞体外培养扩增形成神经球,分别用胰蛋白酶消化法、巴斯德管吹打法和消化振荡法,将神经球打散成单细胞悬液,通过台盼蓝染色测定细胞活性及接种后增生情况,观察3种方法对神经干细胞活性的影响。结果消化振荡法打散神经球,细胞存活率(96.20%)和再次成球率(19.24%)都较其他2种方法高,差异有统计学意义(P<0.05)。结论消化振荡法对人胚胎神经干细胞的活性影响较小,是一种有效、安全的将神经球打散成单细胞悬液的实验方法。

A New Method of Dispersing Neuropheres into Single Cells

Objective To compare the three different methods of dispersing neuropheres of neural stem cells into single cells.Methods The neural stem cells were isolated of 16~20 weeks old human fetus,and were cultured and expanded to form neuropheres.Three different dispersing methods including digesting with Trypsin,triturating with Pasteur pipette and the new method of digesting and shaking were used to disperse neuropheres and the viability and clonability of the resulting cells were analyzed.Results The method of digesting combined with shaking yielded the best survival rate and best proliferation potential of cells.Conclusion This study suggests a valid and safe method that can be used for efficient passage of neural stem cells.