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甲萘威对雌性大鼠血清雌激素水平及抗氧化系统功能的影响
引用本文:邱阳,陈建锋,宋玲,何俊,刘茹,张淳文,王心如. 甲萘威对雌性大鼠血清雌激素水平及抗氧化系统功能的影响[J]. 中华劳动卫生职业病杂志, 2005, 23(4): 290-293
作者姓名:邱阳  陈建锋  宋玲  何俊  刘茹  张淳文  王心如
作者单位:210029,南京医科大学毒理学研究所
基金项目:国家自然科学基金资助项目(30271134);国家基础研究重大项目前期研究专项基金项目(2001CCA04900)(本次实验在研究过程中受到了徐莉春、王玉邦、戴贵东、卞倩、崔仑标、朱正平、孙宏、石远、钱婕等的大力协助,志谢!)
摘    要:目的观察甲萘威对雌性大鼠的生殖毒性并初步探讨其机制。方法雌性SD大鼠经口染毒甲萘威,剂量为0、1.028、5.140、25.704mg·kg-1·d-1。阴道脱落细胞涂片法观察大鼠动情周期的变化;放射免疫法测定其血清雌二醇(E2)、孕酮(P4)水平;分光光度法测定其血清超氧化物歧化酶(SOD)、谷胱甘肽硫转移酶(GST)的活力以及丙二醛(MDA)、谷胱甘肽(GSH)的含量。结果各剂量甲萘威染毒组大鼠动情周期数明显低于对照组。染毒后15d大鼠动情各期出现变化,与对照组的差异有统计学意义(P<0.05,P<0.01)。25.704mg·kg-1·d-1组大鼠体重增长明显低于对照组。各剂量染毒组大鼠的多个脏器系数均明显降低。25.704mg·kg-1·d-1组大鼠血清中E2水平为(19.93±2.21)nmoll,1.028mg·kg-1·d-1组大鼠P4水平为(1.21±0.40)nmoll,与对照组[(28.76±6.12)、(0.63±0.39)nmolL]的差异均有统计学意义(P<0.05)。随染毒剂量增高,大鼠SOD活力在卵巢先降后升,在血清中略升后下降;MDA含量则呈在卵巢中渐升高、在血清中略升后降低趋势;GSH含量和GST活力在卵巢中呈先降后升趋势,但在血清中,GSH含量呈下降趋势,GST活力先上升后下降。结论甲萘威可致雌性大鼠动情周期紊乱及雌激素水平改变,对大鼠的抗氧化系统产生一定影响。

关 键 词:杀虫药 氨甲酸酯 雌激素类 氧化性应激
收稿时间:2004-10-08
修稿时间:2004-10-08

Effects of carbaryl on serum steroid hormone and the function of antioxidant system in female rats
QIU Yang,CHEN Jian-feng,SONG Ling,HE Jun,LIU Ru,ZHANG Chun-win,WANG XIN-RU. Effects of carbaryl on serum steroid hormone and the function of antioxidant system in female rats[J]. Chinese journal of industrial hygiene and occupational diseases, 2005, 23(4): 290-293
Authors:QIU Yang  CHEN Jian-feng  SONG Ling  HE Jun  LIU Ru  ZHANG Chun-win  WANG XIN-RU
Affiliation:Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China.
Abstract:OBJECTIVE: To investigate the effects of carbaryl on serum steroid hormone and function of antioxidant system in female Sprague Dawley rats. METHODS: Carbaryl was administrated to the adult female rats at doses of 0, 1.028, 5.140 and 25.704 mg x kg(-1) x d(-1) for 30 d. Vaginal smears of rats were taken to determine estrous cycle. Serum 17beta-estradiol (E(2)) and progesterone (P(4)) concentrations were measured by radioimmunoassay. The activities of superoxide dismutase (SOD) and glutathione-s-transferase (GST), and the levels of malondialdehyde (MDA) and glutathione (GSH) were measured by spectrophotometry. RESULTS: The number of estrous cycle in exposed groups were obviously lower than in control group (P < 0.05, P < 0.01). Body weight gain in high dose group (25.704 mg x kg(-1) x d(-1)) was significantly lower than that in control. Meanwhile, the organ coefficient of ovary and uterus declined in a dose-dependent manner. Serum E(2) level [(19.93 +/- 2.21) nmol/L] in 25.704 mg group was lower than in control group [(28.76 +/- 6.12) nmol/L, P < 0.05], and P(4) level (1.21 +/- 0.40) nmol/L in 1.028 mg group was higher than that in control group [(0.63 +/- 0.39) nmol/L, P < 0.05]. The activity of SOD first reduced then rose in ovary, and first rose then reduced in serum. The contents of MDA increased in ovary, while decreased in the serum. GSH contents and GST activity increased in ovary, while in serum GSH contents decreased and GST activity first increased then decreased. CONCLUSION: Carbaryl could disrupt estrous cycle and affect serum steroid hormone, and the function of antioxidant system in female SD rats.
Keywords:Insecticides, carbamate    Estrogens    Oxidative stress
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