固本防哮饮对哮喘缓解期小鼠气道杯状细胞增生与黏液分泌相关基因的调控作用

目的?探讨中药复方固本防哮饮对哮喘缓解期小鼠气道杯状细胞增生与黏液分泌相关基因IRE1β、SPDEF、AGR2和mCLCA3的影响。方法?结合前期研究采用卵蛋白(OVA)致敏和OVA-呼吸道合胞病毒(RSV)联合诱导激发BALB/c雌性小鼠，建立哮喘缓解期动物模型，随机分为正常组，模型组，固本防哮饮低、中、高剂量组，孟鲁司特钠组及地塞米松组。qPCR法检测肺组织中IRE1β、SPDEF、AGR2和mCLCA3 mRNA表达水平。结果?模型组小鼠肺组织中IRE1β、SPDEF、AGR2 mRNA表达显著高于正常组(P＜0.05)，而mCLCA3 mRNA表达显著低于正常组(P＜0.05)；固本防哮饮高、中、低剂量组，孟鲁司特钠组及地塞米松组小鼠肺组织中IRE1β、SPDEF、AGR2 mRNA水平较模型组存在不同程度的下降，而固本防哮饮低剂量组和孟鲁司特钠组小鼠肺组织中mCLCA3 mRNA表达显著高于正常组(P＜0.05)。结论?固本防哮饮防治哮喘抑制气道杯状细胞增生和减少黏液分泌的作用机制可能是通过降低IRE1β、SPDEF、AGR2 mRNA表达所致。 

Effects of Guben Fangxiao Decoction on the mRNA Expression of Genes Related to Airway Goblet Cell Hyperplasia and Mucus Hypersecretion in a Murine Asthma Remission Model

OBJECTIVE?To investigate the effects of Guben Fangxiao Decoction on mRNA expression of airway goblet cells hyperplasia and mucus hypersecretion-associated genes like IRE1β， SPDEF， AGR2， and mCLCA3 in mice model with asthma at remission stage. METHODS?Based on the previous study， OVA sensitization combined with RSV sensitization was applied to establish the asthma model on BALB/c female mice， which were randomly divided into the normal group， model group， Guben Fangxiao Decoction of low， middle and high dose groups， montelukast sodium group and dexamethasone group. qPCR was applied to detect the expression of IRE1β， SPDEF， AGR2 and mCLCA3 mRNA in the lung tissue. RESULTS?The expressions of IRE1β， SPDEF and AGR2 mRNA in the lung tissue of the model group were significantly higher than those of the normal group(P<0.05)， while the mCLCA3 mRNA expression was much lower than the normal group(P<0.05). The mCLCA3 mRNA expressions in the lung tissue of Guben Fangxiao Decoction with low， middle and high dosage groups， montelukast sodium group and dexamethasone group experienced decline to different degree. Whereas the mCLCA3 mRNA levels in the Guben Fangxiao Decoction Low dose group and montelukast sodium group were significantly higher than those of the normal group(P<0.05). CONCLUSION?Gubenfangxiao Decoction can significantly attenuate RSV-OVA-induced airway goblet cells hyperplasia and mucus hypersecretion in murine asthma remission model. These effects may be mediated， at least partially， by suppressing the mRNA expression of IRE1β， SPDEF and AGR2.