| 基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究 |
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| 作者姓名： | 孟欣 汪受传 谢彤 徐建亚 沈存思 单进军 |
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| 作者单位： | 1.江苏省儿童呼吸疾病中医药重点实验室，南京中医药大学中医儿科学研究所，江苏 南京?210023 |
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| 摘 要： | 目的?通过气相色谱-质谱联用（GC-MS）技术分析RSV肺炎小鼠尿液和粪便中代谢物的相对含量变化，寻找可协助RSV肺炎诊断的生物标记物。方法?RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型，分别收集小鼠的尿液和粪便，经肟化和衍生化后，运用GC-MS检测尿液和粪便中所含有的代谢物及其相对含量的变化。结果?RSV肺炎小鼠的尿液中共测得38种代谢产物，其中乳酸、丙酸、L-脯氨酸为差异代谢产物（P<0.05）；粪便中共测得43种代谢物，其中亮氨酸、天冬氨酸、胱氨酸、酪氨酸、嘧啶有显著差异（P<0.05）。结论?运用GC-MS代谢组学技术寻找RSV肺炎小鼠尿液和粪便中的生物标记物具有可行性。
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| 关 键 词： | 气相色谱-质谱联用 尿液 粪便 呼吸道合胞病毒肺炎 生物标记物 |
| 收稿时间： | 2016/9/25 0:00:00 |
| 修稿时间： | 2016/12/25 0:00:00 |
Biomarkers in Urine and Feces of BALB/c Mice Infected with RSV Pneumonia Using GC-MS Technology |
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| Authors: | MENG Xin WANG Shou-chuan XIE Tong XU Jian-y SHEN Cun-si SHAN Jin-jun |
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| Institution: | 1.Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, 210023, China2.Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing, 210023, China |
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| Abstract: | OBJECTIVE?To detect the relative abundance changes of metabolites in urine and feces of BALB/c mice to seek the biomarkers that contribute to the diagnosis by GC-MS technology. METHODS?BALB/c mice were challenged intranasally to establish the RSV pneumonia models. Urine and feces were collected respectively， oximated， derivatized and detected by GC-MS. RESULTS?38 metabolites in urine were detected， among which lactic acid， propanoic acid and L-proline were statistically significant (P<0.05). Moreover， 43 metabolites in feces were detected， and leucine， aspartic acid， cystine， tyrosine and pyrimidine had statistical significance(P<0.05). CONCLUSION?GC-MS technology is feasible for seeking biomarkers in urine and feces of mice infected with RSV pneumonia. |
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| Keywords: | GC-MS urine feces RSV pneumonia biomarkers |
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