基于Au_(nano)-PB、AEAPS-Dextran-Fe_3O_4和Au_(nano)修饰的前列腺特异性抗原电流型免疫传感器的研究

目的采用普鲁士兰-纳米金(Au_(nano)-PB)、氨基硅烷化-葡聚糖-四氧化三铁纳米复合物(AEAPS-Dextran-Fe_3O_4)及纳米金(Aunano)共修饰于氧化铟锡(ITO)电极表面固载抗体制得高灵敏前列腺特异性抗原电流型免疫传感器。方法先将ITO电极表面滴加适量Au_(nano)-PB,随后将AEAPS-Dextran-Fe_3O_4滴涂在ITO玻璃电极的Au_(nano)-PB膜上,然后通过静电吸附Au-nano并固定前列腺特异性抗体(anti-PSA),制得电流型免疫传感器。结果在最佳实验条件下,该传感器响应的峰电流值与前列腺特异性抗原(PSA)浓度在该传感器(0.8～20)ng/mL及(20～170)ng/mL的范围内保持良好的线性关系,检测下限为0.6ng/mL。结论成功建立了前列腺特异性抗原免疫传感器,该方法制备简单,操作便捷,检测下限低。

An Immunosensor for Prostate Specific Antigen Based on Aunano-PB, AEAPS-Dextran-Fe3O4 and Aunano

Objective To design a sensitive amperometric immunosensor for the detection of prostate specific antigen（PSA） based on Aunano-PB nanocomposites, AEAPS-Dextran-Fe304 nanocomposites and Audio modified indium tin oxides （ITO） electrode. Methods First the ITO electrode was modified with AUnano-PB nanocomposites. Then AEAPS-Dextran-Fe3O4 nanocomposite was dropped on the Aanano-PB modified-ITO electrode surface. Finally, Aunano was adsorbed onto the composite surface for immobilizing PSA antibodies. Results The optimal Ph value was 7.0,and incubation time was 10 min. In the optimal conditions,the amperometric immunosensor showed a linear increase in the relative intensity at two PSA concentrations of 0.8 to 20ng/mL and 20 to 170ng/mL with a detection limit of 0.6ng/mL. Conclusion The amperometric immunosensor established in this study has been demonstrated to be simple and convenient in the detection of PSA, with low detection limit.