| 大脑皮质星形胶质细胞体外条件化培养体系的建立 |
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| 引用本文： | 马丽娜,赵文静,洛涛,野元裕,古川昭荣. 大脑皮质星形胶质细胞体外条件化培养体系的建立[J]. 吉林大学学报(医学版), 2004, 30(5): 727-729 |
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| 作者姓名： | 马丽娜 赵文静 洛涛 野元裕 古川昭荣 |
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| 作者单位： | 1.吉林大学再生医学科学研究所生物化学与分子生物学实验室，吉林 长春130021；2. 日本岐阜药科大学，日本 岐阜502-8585 |
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| 摘 要： | 目的：建立大脑皮质星形胶质细胞体外条件化培养体系。方法：用快速灵敏的MTT比色法测定细胞活力，对纯化细胞进行形态学观察、免疫组化鉴定。结果：经分离、纯化，获得了纯的单一细胞群，星形细胞MTT测定的A值为0.255±0.012。结论：建立大脑皮质星形胶质细胞体外条件培养体系可以快速、高效得到纯度高的贴壁的星形细胞。
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| 关 键 词： | 方法 星形细胞 |
| 文章编号： | 1671-587X(2004)05-0727-03 |
| 收稿时间： | 2003-03-05 |
| 修稿时间： | 2003-03-05 |
Establishment of culture system of conditionalization for astrocytes of cerebral cortex in vitro |
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| MA Li-na,ZHAO Wen-jing,LUO Tao,NOMOTO Hiroshi,FURUKAWA Shoei. Establishment of culture system of conditionalization for astrocytes of cerebral cortex in vitro[J]. Journal of Jilin University: Med Ed, 2004, 30(5): 727-729 |
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| Authors: | MA Li-na ZHAO Wen-jing LUO Tao NOMOTO Hiroshi FURUKAWA Shoei |
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| Affiliation: | 1. Biochemistry and Molecular Biology Laboratory, Institute of Frontier Medical Sciences, Jilin University,Changchun 130021,China;2. Gifu Pharmaceutical University, Gifu 502-8585,Japan |
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| Abstract: | Objective To establish the culture system of conditionalization for the astrocytes of cerebral cortex in vitro.Methods The cell activity was determined by means of quick and sensitive MTT colorimetry. The purified cells were observed in morphology and identified in immunohistochemistry. Results The pure and single cell groups passing through separation and purify were obtained. The A value of astrocytes detected with MTT was 0.255±0.012.Conclusion The establishment of culture system of conditionalization for the astrocytes of cerebral cortex in vitro can quickly and effectively obtain the high purity astrocytes. |
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| Keywords: | cell culture/methods astrocytes |
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