骨益补对去卵巢大鼠骨质疏松作用的研究

目的：研究骨益补对去卵巢大鼠骨质疏松的作用。方法：去卵巢诱导大鼠骨质疏松症，骨益补以100，200，400mg/kg/d的剂量灌胃给药3月后，以比色法测定其血清钙(s-Ca)、骨碱性磷酸酶(b-AKP)和血清抗酒石酸酸性磷酸酶(s-TRAP)的活性，竞争放射免疫法测定血清中骨钙素(s-BGP)的含量；双能X线骨密度仪(DEXA)测定股骨及腰椎的骨密度；三点弯曲试验法测定骨生物力学；扫描电镜进行骨小梁形态计量学分析。结果：骨益补能增加去卵巢大鼠子宫重量，降低s-TRAP水平，增加股骨骨密度，改善股骨抗弯抗拉能力，改善骨小梁形态，显著增加骨小梁平均宽度(MTT)与骨小梁面积百分比(TS％)。结论：骨益补对去卵巢大鼠骨质疏松症具有防治作用，其机制可能与抑制骨高转换有关。

Studies on Effects of GYB on Osteoporosis in Ovariecto-mized Rats

AIM:To study the effects of GYB(Ext. of Semen cuscutae and fructus cnidii) on osteoporosis in ovariectomized rats METHOD:The osteoposis was induced by ovariectomy Having been fed with GYB,at the dose of 100,200,400 mg/kg/d,to the rats continuously for 3 months,the concentration of calcium(s Ca),tartrate resistant acid phosphatase in serum(s TRAP) and alkaline phosphatease in bone(b AKP) were measured by colorimetric method The concentration of osteocalcin in serum(s BGP) was measured by competitive radio immunologic method Bone mineral density(BMD) of femur and lumbar of rats was determined by Dual Energy X ray Absorbtiometry(DEXA) Biomechanical characteristics of femur were measured by three point bending test Histomorphometry of bone trabecula was analysed by scanning electron microscope RESULT:The BMD of femur of rats and uterus weight were increased The level of s TRAP was decreased The resistance of bending and compression of fumer of rats was enhanced The form of bone trabecula was improved and MTT and trabeculae square percentage (TS%) were increased obviously too CONCLUSION:GYB had antiosteoporotic effects on ovariectomized rats by inhibiting the bone tureover