生肌象皮膏对糖尿病大鼠溃疡创面肉芽组织的终末糖基化产物及受体的影响*

目的]通过对糖尿病大鼠溃疡创面肉芽组织的终末糖基化产物及受体的含量的动态观察,探讨生肌象皮膏促进糖尿病大鼠难治性溃疡愈合的机制.方法]制备糖尿病溃疡动物模型,按照血糖和体质量分层随机分为糖尿病空白对照组、凡士林组、生肌象皮膏组.生肌象皮膏组外用生肌象皮膏纱条外敷,凡士林组用凡士林纱条外敷,空白组、糖尿病对照组均以生理盐水纱条外敷,分别于1、7、14、21 d每组处死动物10只,手术取新鲜肉芽组织160例.采用实时荧光定量PCR法检测终末糖基化产物受体的含量,免疫组化法检测终末糖基化产物的含量.结果]终末糖基化产物的含量于糖尿病各组与空白组比较无显著性差异,均呈强阳性表达.糖尿病各组间比较无统计学意义.糖尿病各组终末糖基化产物受体(RAGE)于7 d有升高的趋势,14d达到高峰,各组间无统计学意义.从均数趋势上看生肌象皮膏组明显低于凡士林组、糖尿病对照组,在均数趋势上看生肌象皮膏组可以抑制RAGE的产生,随时间的延长,更为明显.第21天糖尿病各组的RAGE呈低表达,生肌象皮膏组、凡士林组与空白组比较有统计学意义.糖尿病各组组间比较,无统计学意义.结论]生肌象皮膏对终末糖基化产物无明显影响,可以减少终末糖基化产物受体的产生,随时间的延长,更为明显.

Effect of shengji corium elephatis mastic on the terminal glycosylation production and acceptor of granulation tissue of ulcus raw surface in rats with diabetes

Objection] To dynamic observe the terminal glycosylation production and acceptor of granulation tissue of ulcus raw surface in rats with diabetes and explore the effect mechanism of Shengji corium elephatis mastic on promoting refractoriness ulcer healing in rats with diabetes.Methods] To establish the animal model of diabetes ulcer.The model rats were demixing randomly divided into diabetes control group,control group,Vasogen group,Shengji corium elephatis mastic group.To put to death the rats in one day,seven day,fourteen day and twenty-one day and obtain fresh granulation tissue by using operation method.To determine the terminal glycosylation production content by using RT-PCR and immunohistochemical method.Results] The content of terminal glycosylation production wasn't obviously differentiation on the rats of every diabetes model group and the expression of terminal glycosylation production was powerful masccline.The acceptor of terminal glycosylation production (RAGE) had a elevatory tendency in rats of every diabetes group in seven day and may achieve the peak time in fourteen day.But there weren't obviously differentiation in every group (P>0.05).From the view of mean tendency,the content of RAGE in Shengji corium elephatis mastic group was obviously lower than that in diabetes control and Vasogen group.The Shengji corium elephatis mastic may inhibit the produc-tion of RAGE with the prolongation of time.The expression of RAGE was low in rats of every diabetes group in twenty day.Conclusion] Shengji corium elephatis mastic hadn't obviously influence on the RAGE and may decrease the production of RAGE with the prolongation of time.