65例小儿轮状病毒肠炎免疫反应及肠外损伤的研究

目的探讨小儿轮状病毒肠炎免疫状态及并发肠道外损伤的发生情况,为临床诊治提供参考。方法回顾性分析我院2011年5月至2012年2月收治的确诊为小儿轮状病毒肠炎患儿65例为实验组,选取同期健康体检的患儿65例作为对照组。采用生化法检测患儿肝功能和心肌酶谱;采用ELISA方法检测粪便轮状病毒特异性抗原;采用免疫透射比浊法测定患儿血清中的免疫球蛋白IgG、IgA、IgM。结果 65例小儿轮状病毒肠炎发生肠外损伤以呼吸系统最高为49.23%,其次是肝脏和心脏损伤,分别为20%和18.46%。两组肝功能和心肌酶谱进行比较,差异有统计学意义(P<0.05);两组患者轮状病毒肠炎对血清免疫球蛋白IgA、IgM、IgG变化的比较,差异有统计学意义(P<0.05)。结论小儿轮状病毒肠炎可发生肠道外感染,检测免疫球蛋白、肝功能、心肌酶谱对监测患儿病情变化有重要的临床指导意义。

Study of Parenteral Organs Injury and Immune Status in 65 Cases of Children with Rotavirus Enteritis

Objective To explore pediatric rotavirus enteritis immune status and concurrent extraintestinal injury,to provide a reference for clinical diagnosis and treatment.Methods 65 cases of children with rotavirus enteritis as the experimental group were retrospectively analyzed from May 2011 to February 2012,while 65 healthy children for check-up of the same period were selected as control group.The biochemical method detects liver function and children myocardial enzyme.ELISA method detects feces rotavirus specific antigen,immune transmission method determines in the serum immunoglobulin IgG,IgA,IgM.Results Extraintestinal injury occurrence in 65 cases of rotavirus enteritis:the respiratory system up to 49.23%,followed by the liver damage and heart,with 20% and 18.46%,respectively.The difference of liver function and myocardial enzyme between the experimental group and control was statistically significant(P<0.05);the difference of serum immunoglobulin IgA,IgM,IgG between the two groups was statistically significant(P<0.05).Conclusion Extraintestinal infection may happen in pediatric rotavirus enteritis,detection of immunoglobulin,liver function,myocardial enzyme spectrum have an important clinical significance in monitoring the changes of the disease state.