小鼠诱导性共刺激分子-Ig在小鼠树突状细胞中的表达及其效应研究

目的 获得mICOS-Ig基因修饰的小鼠DC，分析表达产物mICOS-Ig对T细胞活化的影响。方法 采用电转染方法将mICOS-Ig表达载体转入小鼠DC，通过ELISA法检测转染小鼠DC 60和96h以及稳定表达的培养上清；观察mICOS-Ig对同种混合细胞培养反应的影响。结果 ELISA法检测证明将mICOS-Ig表达载体转入小鼠DC，获得瞬时表达及稳定表达，mICOS-Ig可抑制单向混合淋巴细胞反应，抑制率达40％，其效应呈剂量依赖性。结论 获得了稳定表达mICOS-Ig融合蛋白的小鼠DC，mICOS-Ig表达产物对同种细胞刺激的增殖反应有抑制作用。

Expression and effects of mICOS-Ig on mouse dendritic cells

Objective To obtain mouse dendritic cells transfected with mICOS-Ig gene and evaluate effects of mICOS-Ig gene products on T cell activating function.Methods mICOS-Ig expression vector was transferred into mouse dendritic cells by electroporation. ELISA was used to detect the supernatant of mouse dendritic cells at the time of 60 and 96 h after transfection and stable expression, the effects of mICOS-Ig on homologous mixed lymphocyte culture was observed using Balb/c and C57BL/10J mouse as reacting cells and stimulating cells respectively. Results We obtained transient and stable expression after mICOS-Ig expression vector transfected into mouse dendritic cells, mICOS-Ig can inhibit single mixed lymphocyte culture reaction. Conclusion We obtained stable expression after ICOS-Ig expression vector transfected into mouse dendritic cells, and could be further used in other study.