聚酯纤维网的角膜生物相容性实验研究

目的探讨聚酯纤维网植入兔角膜板层后与角膜组织的生物相容性及其与角膜的生物愈合特性。方法选择20只健康新西兰兔,体重2.0～2.5 kg,右眼均行手术作为实验组,左眼作为正常对照组。实验组中15只右眼角膜板层植入聚酯纤维网,并分为术后2周、1个月、3个月、5个月、8个月亚组,作为不同时段的取材期,每亚组3只右眼;其余5只右眼角膜仅制备囊带而不植入材料,作为手术对照亚组;术后定期观察角膜水肿和新生血管生长情况,并取角膜行光镜检查。结果术后早期实验组兔眼不同程度地出现角膜水肿和新生毛细血管的长入,经术后恢复或拆线后均呈自行性消退和闭锁。在最长为8个月的观察期中,植入物在角膜组织中稳定。与植入材料相贴的角膜板层上下界面,成纤维细胞数量明显增加,网孔部位尤其明显,并最终形成胶原纤维紧密的连接。所有植入材料的兔眼均未见角膜坏死、溃疡及植入物脱出。结论聚酯纤维网在兔角膜组织中具有良好的生物相容性。

Biocompatibility of polyester net in the cornea of rabbits

Objective To explore biocompatibility and biohealing characteristics of a polyester net with cornea after the net implanted into the cornea lamina Methods Twenty New Zealand rabbits weighing 2 0 2 5 kg were randomly divided into experimental and control group Surgery was performed in right eye of all these animals and the left one as control A square of implant of 5 6 mm in diameter was cut from polyester net (0 2 mm in thickness and 1 mm pore diameter) and implanted into the cornea lamina of experimental group; only a sack was made without implantation in control group Cornea edema and growth status of new blood vessels were observed at scheduled times after operation; animals were sacrificed 2 weeks, 1, 3, 5 and 8 months after operation and their cornea was removed and examined under light microscopy Results Different degrees of cornea edema and new vessels were revealed after surgery The edema and neovascularization were all automatically disappeared in the period of post operation or after suture removed The implant was stable inside the cornea and lasted as longest as 8 months The number of fibroblasts was markedly increased at the upper and bottom surface of the cornea lamina close to the implant and in the area of net pore particularly And finally, a close connection of collagen fibers was formed No serious complications such as necrosis, ulcer, as well as implant dislocation from cornea were seen Conclusion The polyester net possesses a well biocompatibility with the cornea tissue