Characterization of supercritical fluid extracts of St. John's wort (Hypericum perforatum L.) by HPLC-MS and GC-MS. |
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| Authors: | Christoph Seger Harald R?mpp Sonja Sturm Ernst Haslinger Peter C Schmidt Franz Hadacek |
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| Institution: | Department of Pharmaceutical Technology, Institute of Pharmacy, Leopold-Franzens University, Innsbruck, Austria. chrostoph.seger@uibk.ac.at |
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| Abstract: | Supercritical fluid extracts (carbon dioxide without modifiers) of St. John's Wort (Hypericum perforatum L., Clusiaceae) were analyzed by GC-MS, HPLC-DAD and HPLC-DAD-MS. Besides the dominating phloroglucinols hyperforin (36.5 +/- 1.1%) and adhyperforin (4.6 +/- 0.1%), the extracts mainly contained alkanes (predominantly nonacosane), fatty acids and wax esters. The apolar components tended to accumulate in a waxy phase resting a top of the hyperforin-enriched phase. No components of higher polarity like naphthodianthrones were found. A set of hyperforin oxidation products was detected and tentatively assigned using HPLC-MS. |
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