HIV-1侵染机体多种组织细胞的分子生物学研究

①目的探讨ＨＩＶ－１是否能够侵染及整合在多种组织细胞的ＤＮＡ中。②方法采用套式ＰＣＲ对来自艾滋病病人的肺、脑、肾、肌肉及口腔脱落细胞标本进行ＨＩＶ－１前病毒ｐｏｌ基因的检测，并根据外侧引物ＰＣＲ和套式引物ＰＣＲ敏感性对原始模板数量进行半定量检测。③结果采用套式引物ＰＣＲ可以在艾滋病病人的肺、脑、肾、肌肉及口腔脱落细胞ＤＮＡ标本中检出ＨＩＶ－１前病毒ｐｏｌ基因，而采用外侧引物ＰＣＲ仅在肺、脑细胞ＤＮＡ标本中检出了该基因。④结论ＨＩＶ－１可以侵染机体的多种组织器官并发生整合

MOLECULAR STUDY OF HIV 1 INFECTION IN MULTIPLE TISSUES BY POLYMERASE CHAIN REACTION WITH NESTED PRIMERS

Objective To confirm whether HIV 1 could infect and integrate into cellular DNA of multiple tissues. Methods The nested primers polymerase chain reaction technique was employed to detect the proviral genome of HIV 1 pol gene integrated in cellular DNA of pathologial tissues including lung, brain,liver, kidney, muscle and cast off cells of oral mucosa from a dead AIDS patient. The number of primary template of HIV 1 pol gene integrated in the cellular DNA of multiple tissues was postulated by half quantitative analysis with the routine and nested PCR. Results The proviral genome of HIV 1 pol gene could be detected in cellular DNA of all pathological tissues by nested PCR, while the gene could only be detected in lung, brain and liver tissues by routine PCR. Conclusion The HIV 1 could infect and integrate into multiple tissues without obviously pathological changes.