塞来昔布对子宫内膜癌细胞增生和COX-2 mRNA表达的影响

 【目的 探讨塞来昔布对子宫内膜癌细胞HEC-1-B的作用及对COX-2 mRNA表达的影响。方法 采用细胞培养、MTT试验研究不同浓度的塞来昔布对人类子宫内膜癌细胞HEC-1-B生长的抑制作用，通过RT-PCR法检测塞来昔布作用后COX-2 mRNA表达的变化。结果 塞来昔布能够有效地抑制HEC-1-B细胞的生长，并呈一定的剂量和时间依赖关系，同时能够抑制其COX-2 mRNA表达，20 μmol／L和50 μmol／L塞来昔布对COX-2 mRNA表达的抑制率分别为25.92 ％和50.81％。结论 塞来昔布可能是通过降低COX-2 mRNA的表达而抑制子宫内膜癌细胞的生长。

Effect of celecoxib on endometrial carcinoma cell proliferation and expression of COX-2 mRNA

Objective To investigate the effect of celecoxib on endometrial carcinoma cell HEC-1-B and expression of the COX-2 mRNA. Methods To investigate the inhibition of different concentration Cele－coxib on the growth of human endometrial carcinoma cell HEC-1-B by the method of cell culture and MTT experiments. Detect the changes of expression of the COX-2 mRNA by RT-PCR. Results Celecoxib could effectively inhibit the growth of the HEC-1-B cell in dose and time dependent manner. Celecoxib could inhib－it the expression of the COX-2 mRNA. The inhibition ratio of expression in COX-2 mRNA of 20 μmol/L and 50 μmol/L Celecoxib is 25.92 ％ and 50.81 ％ respectively. Conclusion Celecoxib may inhibit the growth of endometrial carcinoma cell by degrading the expression of the COX-2 mRNA.