创伤性脑水肿的发生机制及Iloprost的治疗作用

目的 探讨创伤性脑水肿的发生机制及前列环素的衍生物Ｉｌｏｐｒｏｓｔ对创伤性脑水肿的治疗作用。方法 干湿重法测定脑组织含水量，甲酰胺法测定伊思蓝（ＥＢ）含量，Ｆｕｒａ－２／ＡＭ荧光标记法测定突触全内「Ｃａ＾２＋」ｉ，并采用前列腺环素稳定的衍生物Ｉｌｏｐｒｏｓｔ进行治疗，研究其对ＥＢ含量、「Ｃａ＾２＋」ｉ及脑水肿的影响。结果 创伤后２ｈ即已发生钙超载，脑组织水含量及ＥＢ含量的增加，Ｉｌｏｐｒｏｓｔ治

Mechanisms of Traumatic Brain Edema and Therapeutic Effect of Iloprost on It

Objective The authors reported the results of the experiment which was carried out in Wistar rats to observe the mechanisms of traumatic brain edema and to evaluate the therapeutic effect of Iloprost on it. Methods The Evens blue(EB) and water contents of brain tissues and the [Ca2 ]i level within the neuronal synapses were determined pre-and post-traumatic brain injury in the rats. These data were then compared with those obtained after administration of Iloprast ( a stable derivative of prostacy-clin). Results Overload of [Ca2 ]i in the neuronal synapses was observed as early as 2h following brain injury. Both the water and EB contents in the brain tissue were also significantly increased as compared with that in the control group. When Iloprast was administrated, the EB content and [Ca2 ]i dropped dramatically and brainedema was significantly relieved. Conclusions Increase in the permeability of blood brain barrier and [Ca2 ]i probably plays a role in the pathogenesis of brain edema after the injury. The authors suggested that Iloprast might have preventive as well therapeutic effects on the patents with traumatic brain injury.