姜黄素通过P53/P21/PCNA/eIF4E信号通路抑制A549细胞增殖

目的研究姜黄素对A549细胞增殖的抑制作用,并探讨其可能的分子机制。方法不同浓度姜黄素处理A549细胞后,分别用平板克隆形成实验、MTT法检测A549细胞的克隆形成数和增殖。20μmol/L姜黄素处理A549细胞24、48、72 h后,FCM检测细胞周期;RT-PCR、Western blot检测P53、P21、PCNA及eIF4E mRNA和蛋白的表达水平。结果 20μmol/L姜黄素可引起A549细胞S期阻滞,抑制细胞增殖,减少细胞的克隆形成数。20μmol/L姜黄素作用A54924 h后P53、P21 mRNA和蛋白表达上调,48 h或72 h后变得尤明显。此外,20μmol/L姜黄素处理A549细胞48 h后可引起PCNA、eIF4E mRNA和蛋白表达水平下调,72 h后尤明显。结论姜黄素可抑制A549的克隆形成数和细胞增殖,其机制可能与姜黄素促进P53和P21基因表达,抑制PCNA和eIF4E基因表达有关。

Curcumin inhibits on proliferation in A549 cells through P53/P21/PCNA/eIF4E signaling pathway

Objective To investigate the inhibitory effect of curcumin on the proliferation in A549 cells and to explore possible mechanisms.Methods After A549 cells were treated with different concentrations of curcumin,cell clone formation and proliferation were determined by colony formation assay and MTT assay,respectively.A549 cells were treated with 20 μmol/L curcumin for 24 h,48 h and 72 h,respectively and the cell cycle was measured by flow cytometry(FCM).mRNA and protein levels of P53,P21,PCNA and eIF4E were detected by RT-PCR and Western blotting.Results A549 cells treated with 20 μmol/L curcumin showed an arrest of cell cycle at S phase,inhibition of cell proliferation and reduction of clone formation;the treatment for 24 h resulted in an increased mRNA and protein levels of p53 and p21,which became more obvious after 48 h and 72 h treatment.In addition,the cells treated with 20 μmol/L curcumin for 48 h showed decreased expressions of eIF4E and PCNA at mRNA and protein levels,and the decrease became more obvious after 72 h treatment.Conclusion Curcumin can inhibit clone formation and cell proliferation in A549 cells.The mechanism may be related to up-regulation of P53 and P21 and down-regulation of PCNA and eIF4E.