表没食子儿茶素没食子酸酯增强低剂量顺铂对小细胞肺癌H446细胞株的细胞毒作用

目的探讨表没食子儿茶素没食子酸酯[(-)-epigallocatechin-3-gallate,EGCG]联合顺铂(Cisplatin,DDP)对小细胞肺癌细胞株H446的细胞毒作用及其机制。方法采用MTT法检测EGCG及DDP影响H446细胞增殖的量效和时效关系。采用流式细胞仪检测不同浓度EGCG、DDP及二者联合作用于H446细胞对细胞周期及凋亡的影响。结果 EGCG和DDP均可抑制H446细胞增殖,该增殖抑制作用呈浓度及时间依赖模式。不同浓度的EGCG(80、160μmol/L)与DDP(1.0μg/ml)联合应用时,可使H446细胞阻滞于G2/M期。EGCG可诱导H446细胞凋亡,并能增强DDP诱导H446细胞凋亡的作用。结论 EGCG能够抑制H446细胞增殖,诱导H446细胞凋亡,增强DDP诱导H446细胞凋亡的作用,其机制可能与G2/M期阻滞有关。

(-)-epigallocatechin-3-gallate enhances low-dose cisplatin cytotoxicity to human small cell lung cancer cell line H446

Objective To determine the effect of(-)-epigallocatechin-3-gallate(EGCG) combined with cisplatin(DDP) on the cytotoxicity,apoptosis,and cell cycle in human small cell lung cancer cell line H446 cells.Methods Human small cell lung cancer H446 cells were treated with different concentrations of EGCG and DDP.The proliferation of cells was assayed by MTT assay.Flow cytometry(FCM) was used to detect cell cycle arrest.Cell apoptosis was analyzed by FCM with Annexin V/PI staining.Results Treatment of EGCG led to a significant inhibition in the proliferation of H446 cells in a dose-and time-dependent manner.The percentage of cells at G2/M phase was increased markedly after treatment with EGCG and low-dose DDP.The apoptosis of H446 cells was induced by EGCG and low-dose DDP.The apoptotic rate was higher in the group of EGCG combined with DDP than in either of them alone.Conclusion EGCG can effectively inhibit the proliferation of H446 cells and induce cell apoptosis.Moreover,EGCG enhances H446 cells apoptosis induced by low-dose of DDP,which may be due to the cell cycle arrest at G2/M phase.