补肾活血方对大鼠心脏成纤维细胞增殖及胶原表达的影响

摘要：目的探讨补肾活血方对大鼠成纤维细胞增殖及胶原表达的影响，为其抗心肌纤维化提供实验依据。方法通过血清药理
学方法制备补肾活血方含药血清，与DMEM配成细胞培养液。提取大鼠心脏成纤维细胞（CFs）并传代培养，将3~4代CFs分为
4组：A组为10%含药血清培养液培养，B组为20%含药血清培养液培养，C组为不含药血清培养液培养，D组为对照组，为常规胎
牛血清培养液培养。培养72 h后CCK-8检测CFs增殖，RT-PCR检测胶原基因表达，Western blotting检测胶原蛋白表达。结果
与C组、D组相比，A组、B组CFs增殖明显降低，B组较A组进一步降低（P<0.05）；Ⅰ型和Ⅲ型胶原mRNA在A、B两组表达较C、
D两组明显下调（P<0.05），Ⅰ型、Ⅲ型胶原蛋白表达在A、B两组明显低于C、D两组（P<0.05）。结论补肾活血方可以抑制大鼠
成纤维细胞增殖及胶原表达。

Effects of Bushen Huoxue Fang on rat cardiac fibroblast proliferation and collagen production in vitro

Objective To investigate the effects of Bushen Huoxue Fang on the proliferation of rat cardiac fibroblasts and collagen production in the cells.Methods Rat cardiac fibroblasts were isolated and cultured in DMEM containing 10%(group A) or 20%(group B) or no(group C) serum from rats treated with Bushen Huoxue Fang,with cells cultured in DMEM containing 10% FBS as the control(group D).After 72 h of cell culture,the proliferation of the fibroblasts was detected using CCK-8 kit,and collagen mRNA and protein expressions were examined using RT-PCR and Western blotting,respectively.Results Compared with that in groups C and D,the cell proliferation decreased significantly in groups A and B,and especially in the latter(P<0.05).RT-PCR demonstrated significant reductions of the mRNAs of type 1 and 3 collagens in groups A and B(P<0.05),and their protein levels were also significantly lowered(P<0.05).Conclusion Bushen Huoxue Fang can effectively inhibit the proliferation of rat cardiac fibroblasts and reduced collagen type 1 and 3 productions in the cells in vitro.