| 博尔纳病病毒对人少突胶质细胞增殖与凋亡的影响 |
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| 引用本文： | 邓婧,李丹,张亮,黄荣忠,李文娟,马丽华,房亮,谢鹏. 博尔纳病病毒对人少突胶质细胞增殖与凋亡的影响[J]. 第三军医大学学报, 2012, 34(5): 401-405 |
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| 作者姓名： | 邓婧 李丹 张亮 黄荣忠 李文娟 马丽华 房亮 谢鹏 |
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| 作者单位： | 邓婧 (400016重庆,重庆医科大学附属第一医院神经内科) ； 李丹 (400016重庆,重庆医科大学神经科学研究中心,重庆市神经生物学重点实验室) ； 张亮 (400016重庆,重庆医科大学神经科学研究中心,重庆市神经生物学重点实验室) ； 黄荣忠 (400016重庆,重庆医科大学附属第一医院神经内科) ； 李文娟 (400016重庆,重庆医科大学神经科学研究中心,重庆市神经生物学重点实验室) ； 马丽华 (400016重庆,重庆医科大学附属第一医院神经内科) ； 房亮 (400016重庆,重庆医科大学神经科学研究中心,重庆市神经生物学重点实验室) ； 谢鹏 (400016重庆,重庆医科大学附属第一医院神经内科) ； |
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| 基金项目： | 国家重点基础研究发展计划(973计划,重庆市自然科学基金,重庆市卫生局医学科学技术研究项目 |
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| 摘 要： | 目的 探讨博尔纳病病毒(Borna disease virus,BDV)感染对人少突胶质细胞(oligodendrocytes,OL)增殖与凋亡的影响.方法 用BDV感染OL细胞,免疫荧光检测OL细胞中博尔纳病病毒P40蛋白的表达,CCK8测定法观察细胞的生长增殖情况,用流式细胞技术检测细胞的凋亡情况和周期变化,Wes...
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| 关 键 词： | 博尔纳病病毒 少突胶质细胞 细胞增殖 细胞凋亡 |
Borna disease virus inhibits proliferation and induces apoptosis in human oligodendrocyte in vitro |
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| Deng Jing,Li Dan,Zhang Liang,Huang Rongzhong,Li Wenjuan,Ma Lihua,Fang Liang,Xie Peng. Borna disease virus inhibits proliferation and induces apoptosis in human oligodendrocyte in vitro[J]. Acta Academiae Medicinae Militaris Tertiae, 2012, 34(5): 401-405 |
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| Authors: | Deng Jing Li Dan Zhang Liang Huang Rongzhong Li Wenjuan Ma Lihua Fang Liang Xie Peng |
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| Affiliation: | 1,2(1Department of Neurology,First Affiliated Hospital;2 Center of Neuroscience,Chongqing Key Laboratory of Neurobiology,Chongqing Medical University,Chongqing,400016,China) |
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| Abstract: | Objective To explore the effect of Borna disease virus(BDV) infection on the proliferation and apoptosis in human oligodendrocytes(OL cells).Methods BDV infection of OL cells was detected by P40 immunofluorescence,cell growth was observed by CCK8 assay,and the apoptosis and cell cycle were detected by flow cytometry.Relative expression levels of Bax and Bcl-2 were also detected by Western blotting.Results Compare to negative control cells,the expression of P40 protein was positive in the experimental cells in 3 d after BDV infection.As the infection time increased,the proliferation of OL cells were decreased.Flow cytometry indicated that BDV infection resulted in an increase of apoptotic cells in 3 d after infection.BDV infected cells were arrested at G2 phase,and cell proliferation was inhibited.Expression of pro-apoptotic protein Bax was increased,and that of antiapoptotic protein Bcl-2 was decreased.Conclusion BDV infection inhibits cell proliferation,and induces cell apoptosis through up-regulating Bax expression and down-regulating Bcl-2 expression in OL cells. |
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| Keywords: | Borna disease virus oligodendrocyte cell cell proliferation cell apoptosis |
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