血管内皮生长因子C及其受体3mRNA在上皮性卵巢癌组织的表达及意义

目的 探讨血管内皮生长因子C（VEGF-C）及其受体3（VEGFR-3）mRNA在上皮性卵巢癌组织的表达及与癌周淋巴管生成及淋巴转移的关系。方法 2003-04—2004-06第三军医大学附属西南医院采用RT-PCR方法，检测VEGF-C及VEGFR-3mRNA在良、恶性上皮性卵巢肿瘤组织的表达及组织化学淋巴管染色并计数，分析其与VEGF-C mRNA表达及淋巴转移的关系。结果 VEGF-C mRNA和VEGFR-3 mRNA在卵巢良、恶性肿瘤的表达差异有显著性。VEGF-C mRNA表达阳性及有淋巴结转移组的癌组织淋巴管密度分别大于VEGF-CmRNA表达阴性及无淋巴结转移组的癌组织，二者差异均有显著性。结论 VEGF-CmRNA在上皮性卵巢癌组织的表达上调导致了癌周淋巴管生成，促进了肿瘤的淋巴道转移。

The mRNA expression of vascular endothelial growth factor C and its receptor 3 in human epithelial ovarian carcinoma

Objective To study the mRNA expression of vascular endothelial growth factor C(VEGF-C) and its receptor 3 (VEGFR-3),in addition the relationship between them and the peri-tumor lymphangiogenesis,lymphatic metastasis in human epithelial ovarian carcinoma was also studied. Methods The expression of VEGF-C mRNA and VEGFR-3mRNA in benign and malignant epithelial ovarian tumors was detected by RT-PCR. Analyse the relationship between the lymphatic vessel density and the expression of VEGF-CmRNA and lymphatic metastasis by enzyme-histochemistry staining and lymphatics counting. Results The expression of VEGF-CmRNA and VEGFR-3mRNA was significantly higher in ovarian carcinomas than those in benign ovarian tumors. Lymphatic density in VEGF-CmRNA positive group and lymphatic metastasis group was significantly higher than those in VEGF-CmRNA negative group and non-lymphatic metastasis group respectively.Conclusion Up-regulation of VEGF-CmRNA in epithelial ovarian carcinoma facilitates the peri-tumor lymphangiogenesis and lymphatic metastasis.