HPLC法测定石见穿中丹酚酸B的含量

目的:建立测定石见穿中丹酚酸B含量的方法。方法:采用高效液相色谱法。色谱柱为Thermo C18(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸水溶液(21∶79,V/V),流速为1.0 ml/min,检测波长为286 nm,柱温为23℃。结果:丹酚酸B的进样量在0.153³.060μg范围内与其峰面积积分值呈良好线性关系(r=0.999 8);精密度、稳定性、重复性试验的RSD<1%;平均加样回收率为97.70%,RSD=0.56%(n=6)。结论:该方法操作简便、准确可靠、重复性好,可作为石见穿药材的质量控制方法。

Content Determination of Salvianolic Acid B in Salvia chinensis by HPLC

OBJECTIVE： To establish the method for the content determination of salvianolic acid B in Salvia chinensis. METHODS： HPLC method was adopted. The determination was performed on Thermo C18（150 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-0.1% formic acid solution（21 ： 79, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 286 nm,and the column temperature was 23 ℃. RESULTS：The linear range of salvianolic acid B were 0.153-3.060 μg（r=0.999 8） with an average recovery of 97.70% （RSD=0.56%, n=6）; RSD of precision, stability and reproducibility tests were lower than 1%. CONCLUSIONS： The method is simple, accurate, reliable, repeatable and can be used for quality control of S. chinensis.