二甲基苯蒽诱发家兔卵巢肿瘤及其生物学特性的初步观察

目的以二甲基苯蒽(DMBA)原位埋线法诱发家兔卵巢肿瘤,并初步观察其生物学特性.方法棉线浸入加热熔化的DMBA中,取雌性家兔5只,分别在其双侧卵巢埋入含有DMBA的棉线,连续观察8个月,处死荷瘤家兔,通过大体、光镜、电镜以及流式细胞技术等方面进行肿瘤生物学特性的初步观察.结果 1只家兔因术后感染而死亡,2只观察8个月无肿瘤发生.2只分别发生未分化癌及腺癌,组织学特点与相应的人卵巢肿瘤很相似;流式细胞分析S期细胞含量分别为40.72%和29.87%.结论二甲基苯蒽能原位诱发较大型动物家兔的卵巢肿瘤,可用于人卵巢癌的相关实验研究.

Establishment and preliminary observation of 7,12-dimethylbenz[a]anthracene-induced ovarian tumor in rabbits

Objective To establish an ovarian tumor model in rabbits with 7,12-dimethylbenza]anthracene(DMBA) and observe its biological features. Methods DMBA was heated, where in the central portion of a cotton yarn was immersed in it. The DMBA-coated yarn was inserted into the ovaries of 5 rabbits. The animals sacrificed after 8 months were observaed. Studies were proceed through microscope, electron microscope(EMS), and flow cytometer (FCM). Results One rabbit was died of severe infection after operation, 2 rabbits were still tumor-free after 8 months, while 2 were formed undifferentiated carcinoma and adenocarcinoma,respectively. It is quite similar with ovarian tumor of woman in biological features. The S-phase of each tumor was 40.72% and 29.87% separately. Conclusion DMBA can induce ovarian tumor in rabbits and it can serve as a big animal model for the study of ovarian tumor.