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| 健康人携带的沙门菌中沙门菌基因组岛1的鉴定及特性分析 | |
| 引用本文： | 赵红波,赵建宏,孙素菊,徐保红,闫鹤,赵红丽,李继红,时东彦,刘晓雷,石磊. 健康人携带的沙门菌中沙门菌基因组岛1的鉴定及特性分析[J]. 分子诊断与治疗杂志, 2010, 2(4): 231-236 |
| 作者姓名： | 赵红波 赵建宏 孙素菊 徐保红 闫鹤 赵红丽 李继红 时东彦 刘晓雷 石磊 |
| 作者单位： | 1. 河北医科大学第二医院临床微生物室,河北省临床检验中心,河北,石家庄,050000 2. 河北医科大学公共卫生学院,河北,石家庄,050017 3. 石家庄市疾病控制中心,河北,石家庄,050011 4. 华南理工大学轻工与食品学院,广东,广州,510640 5. 河北省武警总队医院 |
| 基金项目： | 国家自然科学基金，科技部国家科技基础平台工作重点项目，教育部科学基金，珠海科学技术规划项目 |
| 摘 要： | 目的 检测健康人携带的沙门菌中沙门菌基因组岛（SGI1）的分布情况及特性,分析其与细菌耐药性的关系.方法 采用PCR方法对5株第一类整合子阳性的沙门菌检测SGI1,分别检测其左侧、右侧部分和其中间的部分基因,以及环状形式和切除状态,通过接合转移及自然转化检测其可移动性.结果 一株鼠伤寒沙门菌携带SGI1,位于thdF和int2之间,且其多重耐药区含aadA2,floR,tetG,blaPSE-1基因,存在环状形式及切除状态,且可通过自然转化方式转移至其他菌株.结论 SGI1可通过自然转化方式转移到其他菌株,介导多重耐药的传播. |
| 关 键 词： | 鼠伤寒沙门菌 沙门菌基因组岛 抗生素耐药 自然转化 |
Identification and characterization of Salmonella genomic island 1 among Salmonella strains from healthy persons |
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| ZHAO Hongbo,ZHAO Jianhong,SUN Suju,XU Baohong,YAN He,ZHAO Hongli,LI Jihong,SHI Dongyan,LIU Xiaolei,SHI Lei. Identification and characterization of Salmonella genomic island 1 among Salmonella strains from healthy persons[J]. Journal of Molecular Diagnosis and Therapy, 2010, 2(4): 231-236 | |
| Authors: | ZHAO Hongbo ZHAO Jianhong SUN Suju XU Baohong YAN He ZHAO Hongli LI Jihong SHI Dongyan LIU Xiaolei SHI Lei |
| Affiliation: | ZHAO Hongbo1, ZHAO Jianhong＊ , SUN Suju2, XU Baohong2, YAN He4, ZHAO Honglil, LI Jihong1, SHI Dongyan, LIU Xiaolei, SHI Lei4 ( 1.Department of Clinical Microbiology, The Second Hospital of Hebei Medical University; Hebei Provincial Centre for Clinical Laboratory Science, Hebei, Shijiazhuang 050000, China; 2. College of Public Health, Hebei Medical University, Hebei, Shijiazhuang 050017, China; 3. Center for Disease Control of Shijiazhuang, Hebei, Shijiazhuang 050011, China; 4.College of Light Industry and Food Sciences, South China University of Technology, Guangdong, Guangzhou 510640, China ) |
| Abstract: | Objective To identify and characterize Salmonella genomic island 1 （SGII） in Salmonella strains--from healthy person, and to analyze the relationship between SGI1 and antibiotic resistance. Methods Five class 1 integron positive strains were tested for the presence of SGI1 with PCR method, and the positive amplicons were sequenced and analyzed. To detect the mobilization of the SGI1, the extrachromosome circle forms or the excision as well as conjugation and natural genetic transformation were examined. Results SGI1 was detected in a Salmonella enterica serovar Typhoid murine strain, with the aadA2, floR, tetG, and blapsE_l genes in its multidrug-resistant region, and it was located between thdF and a retronphage （int2）. Both the extrachromosome circle form of SGI1 and the excision of SGI1 were detected, and it could transfer through natural genetic transformation. Conclusion The presence of SGII in Salmonella strains from healthy person suggests that widespread surveillance of antibiotic resistance should be conducted at the gene level. |
| Keywords: | Salmonella enterica serovar Typhimurium Salmonella genomic island 1 Antibiotic resistance Natural genetic transformation |
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