毫米波辐照对成年子鼠学习记忆功能影响及机制的探讨

为阐明毫米波辐射是否会导致成年子鼠学习记忆功能改变及其机理。用频率３６．１１ＧＨｚ、功率密度１０．０ｍＷ／ｃｍ２的毫米波，在小鼠怀孕６ｄ～１５ｄ时进行２ｈ／ｄ辐射，用Ｙ型电迷宫对成年子鼠进行学习记忆功能测试，分别用受体的放射配基结合分析（ＲＢＡ）、放射免疫（ＲＩＡ）、高效液相色谱－电化学检测（ＨＰＬＣ－ＥＣＤ）对仔鼠脑胆碱能－Ｍ受体（Ｍ－Ｒ）、精氨酸加压素（ＡＶＰ）和生长抑素（ＳＳ）、单胺类递质的含量进行测定，对仔鼠不同脑区进行光镜和电镜观察。结果显示：毫米波辐射可导致成年子鼠学习记忆成绩降低、足孕胚胎垂体内和成年子鼠下丘脑中ＡＶＰ含量明显降低、足孕胚胎和成年子鼠下丘脑、垂体中ＳＳ含量明显升高、成年子鼠海马区Ｍ－Ｒ数量明显升高、足孕胚胎、成年子鼠及孕鼠脑中多巴胺（ＤＡ）含量明显降低，但未导致仔鼠脑在结构上发生明显改变。认为：毫米波辐射导致成年子鼠学习记忆功能降低与使其脑内Ｍ－Ｒ、ＡＶＰ、ＳＳ及ＤＡ改变相关

Effects of Millimeter Wave Irradiation on the Learning Capacity Memory of the Adult Posterity and Its Mechanism

The purpose of our study is to clarify whether millimeter wave irradiation will results in alterations in learning capacity, memory of the adult posterity and its mechanism.Two month old BALB/c mice were irradiated by millimeter wave with a frequency of 36 11 GHz at a power density of 10 0 mW/cm 2 for 2h daily from the 6th to 15th day of gestation.The tests of learning capacity and memory of adult posterity were employed on Y type electric maze.The quantity of M R in the embryos′ whole brain and different parts of the brain of adult posterity and its affinity to ligand, the contents of AVP and SS in hypothalamus and pituitary gland of the embryos at term and adult posterity, and the contents of monoamine transmitter and main metabolic in brain of the embryos at term, adult posterity and pregnant mice were measured by using RBA, RIA and HPLC ECD separately.The embryos′ brain at term, the different parts of adult posterity brain were observed under microscope and electron microscope.Results revealed that: millimeter wave irradiation lowered learning capacity and memory of adult posterity.It significantly reduced the content of AVP in pituitary gland of the embryos at term and in hypothalamus of adult posterity.Meanwhile, it significantly increased the content of SS in pituitary gland and hypothalamus of the embryos at term and adult posterity.It obviously increased quantity of M R in hippocampus of adult posterity.It obviously reduced the content of DA in brain of the embryos at term, adult posterity and pregnant mice.It caused no structural changes in the brains of the embryos at term and the adult posterity.It suggested that the mechanism for lowering learning capacity and memory of the adult posterity is closely related to the content changes of M R, AVP, SS and DA in brain of the posterity.