大鼠肢体缺血再灌注对脊髓神经元细胞凋亡的影响及机制探讨

目的：观察在大鼠肢体缺血再灌注脊髓神经元损伤的过程中，细胞凋亡以及Bcl-2、Caspase-3蛋白质表达的变化。方法：复制实验性大鼠肢体缺血再灌注损伤模型，观察脊髓L1-5节段的病理学改变，TUNEL法检测细胞凋亡情况，免疫组化检测脊髓神经元Bcl-2、Caspase-3蛋白质表达的变化。结果：肢体缺血再灌注后Bcl-2、Caspase-3表达明显增多，Bcl-2在12h达高峰，之后下降；Caspase-3在24h达高峰，24h的凋亡细胞数最多，Caspase-3的表达与凋亡细胞数呈正相关关系，r=0．946。结论：肢体缺血再灌注后出现脊髓神经元损伤，并有细胞凋亡发生。

Effects and Its Mechanisms of Limbs Ischemia-Reperfusion in Rats on the Cell Apoptosis of Spinal Cord Neurons

Objective：To observe the expressive changes of the cell apoptosis, Bcl-2 and Caspase-3 during the process of spinal cord neuron injury of limbs isehemia-reperfusion （LIR） in rats. Methods：The limbs ischemia-reperfusion injury model of experimental rats was duplicated to observe the pathologic changes of the segment L1-5 of the spinal cord by HE staining, to detect the cell apoptosis by TUNEL method and to determine spinal cord neurons Bcl-2 and Caspase-3 protein by immunohistochemistry. Results：The expression of Bcl-2 and Caspase-3 followed LIR was significantly increased. Bcl-2 got the peak value at 12h and then was gradually decreased. Caspase-3 got the peak value at 24h and the numbers of cell apoptosis were the most at 24h. The expression of Caspase-3 and the numbers of cell apoptosis were positive correlation and r = 0.946. Conclusion： Spinal cord neuron injury may appear after limbs ischemia-reperfusion（LIR） and the cell apoptosis may occur.