JNK和Caspase-3在夏枯草引起人淋巴瘤细胞凋亡中的作用

目的 研究夏枯草对淋巴瘤细胞生长的影响及其可能的机制.方法 参考临床常用剂量,采用50 g/L夏枯草(夏枯草组)、50 g/L夏枯草及20 μmol/L c-Jun氨基末端激酶(JNK)特异抑制剂SP600125(SP600125组)处理Raji细胞,以加入同体积生理盐水处理的Raji细胞作对照,应用噻唑蓝比色法(MTT法)检测各处理方式下细胞增殖率,通过Western印迹检测JNK、c-Jun磷酸化水平和半胱氨酰天门冬氨酸(Caspase-3)的表达,流式细胞术(FCM)检测细胞凋亡率.结果 夏枯草细胞增殖率(67.32±1.96)%低于其余各组(P<0.05);JNK磷酸化水平(0.48 ±0.03)和c-Jun磷酸化水平(0.46±0.04)在夏枯草组均明显增高(P<0.05),SP600125可抑制c-Jun磷酸化(0.43±0.01);夏枯草组的Caspase-3表达(1.35±0.07)高于其他各组,SP600125使其表达减少(0.79±0.06,P<0.05);细胞凋亡率在夏枯草组中最高(25.32±5.27)%(P<0.05).结论 夏枯草可以明显抑制Raji细胞的生长,这种抑制作用可能是通过激活JNK信号转导通路和Caspase途径导致细胞凋亡实现的.

Involvement of JNK and caspase-3 in human lymphoma cell apoptosis induced by Prunella vulgaris

Objective To characterize the effect of Prunella vulgaris upon lymphoma cells and its possible mechanisms.Methods Raji cells were subjected to different therapeutic schemes:Prunella vulgaris(50 g/L)group,P.vulgaris(50 g/L)+SP600125(20 μmol/L,JNK inhibitor)(SP600125 group):Raji cells in physiological saline served as the control.MTT assay was used to measure the cellular proliferation.Phosphorylation of JNK,c-Jun and the expressions of Caspase-3 were determined by Western blot.Cell apoptosis was analyzed by FCM.Results The proliferation rate of cells in the P.vulgaris group (67.32±1.96)% was significantly lower than that in the other groups (P<0.05).The phosphorylation of JNK(0.48±0.03)and the phosphorylation of c-Jun(0.46±0.04)significantly increased in the P. vulgafis group(P<0.05)and SP600125 inhibited the phosphorylation of c-Jun(0.43±0.01).The expression of Caspase-3 in the P.vulgaris group (1.35±0.07) was higher than that in the other groups.And such an effect could be blocked by SP600125(0.79±0.06) (P<0.05).The rate of cellular apoptosis was the highest in the P.vulgaris group(25.32±5.27)% (P<0.05).Conclusion P.vulgaris shows a strong inhibitory effect upon the growth of lymphoma cell line Raji probably through the activation of JNK pathway and caspase channel and then apoptosis.