| HPLC法测定感冒退热颗粒中腺苷的含量 |
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| 引用本文： | 赵成.HPLC法测定感冒退热颗粒中腺苷的含量[J].现代中药研究与实践,2008,22(4):28-29. |
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| 作者姓名： | 赵成 |
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| 作者单位： | 安徽省六安市药品检验所 |
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| 摘 要： | 目的建立HPLC法测定感冒退热颗粒中腺苷的含量，以有效控制其内在质量。方法采用VP-ODS柱（150mm×4．6mm，5μm）；磷酸盐缓冲液（pH6．5）-甲醇（17：3），检测波长260mm，流速1．0mL·min^-1，柱温为室温。结果腺苷在3．93-39．28mg·L^-1（r=0．9999，n=6）范围内线性关系良好，平均回收率为99．3％，RSD=0．61％。结论本法简便、灵敏、重复性好、准确可靠，可用于感冒退热颗粒中腺苷的测定。
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| 关 键 词： | 感冒退热颗粒 HPLC法 腺苷 含量测定 |
Determination of Adenosine in Ganmao Tuire Granules by HPLC |
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| ZHAO Cheng.Determination of Adenosine in Ganmao Tuire Granules by HPLC[J].Research and Practice on Chinese Medicines,2008,22(4):28-29. |
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| Authors: | ZHAO Cheng |
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| Institution: | ZHAO Cheng ( Luan Institute for Drug Control , Luan 237000, China ) |
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| Abstract: | Objective To establish a method for the determination of adenosine in Ganmao Tuire granules.Methods The samples were separated by VP-ODS column(150 mm×4.6 mm,5 μm),the mobile phase was phosphate acid buffer solution(pH 6.5)-methanol(17∶3),the detection wavelength was at 260 nm,flow rate was 1.0 mL·min-1,the column temperature was indoor temperature.Results The linear range of adenosine was in 3.93～39.28 mg·L-1(r=0.999 9,n=6).The average recovery was 99.3% with RSD of 0.61%.Conclusion This method is simple,sensitive,accurate and can be used to determine the contents of adenosine in Ganmao Tuire gralunes. |
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| Keywords: | Ganmao Tuire granules HPLC adenosine determination |
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