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正交试验法优选复方降糖方提取工艺
引用本文:毛蕾,何岚,卢婉妃,袁强.正交试验法优选复方降糖方提取工艺[J].中华中医药学刊,2011(11):2519-2521.
作者姓名:毛蕾  何岚  卢婉妃  袁强
作者单位:浙江中医药大学药学院;浙江中医药大学附属第二医院;
基金项目:浙江省科技厅社会发展重点项目(2007C23019)
摘    要:目的:对复方降糖方提取工艺进行探讨。方法:水提取工艺以多糖得率为考察指标,醇提取工艺以栀子苷为考察指标,分别采用正交设计法对其最佳提取工艺条件进行优选。结果:6倍量水回流提取3次,每次2h,提取液合并浓缩至1g/mL,醇沉浓度为90%,放置时间24h为最佳水提取工艺条件;醇提取最佳工艺条件为:药材加65%乙醇10倍量提取2次,每次2h。结论:试验结果为确定复方降糖方的提取工艺提供了试验依据。

关 键 词:复方降糖方  提取工艺  正交实验

Optimization of Extracting Technology for Compound Jiangtang Hypoglycemic Recipe by Orthogonal Test
MAO Lei,HE Lan,LU Wan-fei,YUAN Qiang.Optimization of Extracting Technology for Compound Jiangtang Hypoglycemic Recipe by Orthogonal Test[J].Chinese Archives of Traditional Chinese Medicine,2011(11):2519-2521.
Authors:MAO Lei  HE Lan  LU Wan-fei  YUAN Qiang
Institution:MAO Lei1,HE Lan2,LU Wan-fei1,YUAN Qiang2(1.College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China,2.The Second Affiliated Hospital of Zhejiang University of TCM,Hangzhou 310005,China)
Abstract:Objective:To study the extracting technology condition for Compound Jiangtang Hypoglycemic Recipe.Methods:Total Polysaccharid in the water extration was the index and the content of Geniposide in ethanol extration by the orthogonal test was the index.Results:The optimum condition of water extraction were 6 times amount of water,decocting triple for 2h each time,with the concentration of alcohil precipitation 90% for 24 hours and optimal ethanol extracted conditions were adding the amount of 10 times 65% eth...
Keywords:compound Jiangtang hypoglycemic recipe  extracting technology  orthogonal test  
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