| 大孔树脂制备岩柏草总黄酮及其含量测定 |
| |
| 引用本文： | 方红英,庄让笑,王福根,席建军,张袆奇,缪樑斌. 大孔树脂制备岩柏草总黄酮及其含量测定[J]. 中华中医药学刊, 2011, 0(12): 2796-2798 |
| |
| 作者姓名： | 方红英 庄让笑 王福根 席建军 张袆奇 缪樑斌 |
| |
| 作者单位： | 浙江中医药大学附属第六医院药剂科; |
| |
| 摘 要： | 目的:探索D-101大孔树脂分离纯化岩柏草总黄酮的最优工艺,并对有效成分穗花杉双黄酮进行含量测定。方法:紫外分光光度法测定总黄酮浓度,以黄酮收率、纯度等为指标,上样质量浓度、吸附流速和洗脱剂乙醇体积分数为影响因素,确定大孔树脂分离纯化岩柏草总黄酮工艺,并用高效液相色谱法测定总黄酮中穗花杉双黄酮。结果:大孔树脂对岩柏草总黄酮有较好的分离效果,其最优工艺条件为上样质量浓度8.5mg/mL,吸附流速为2mL/min,乙醇体积分数为70%,总黄酮得率为0.89%。流动相:乙腈-0.5%冰醋酸溶液,梯度洗脱;检测波长:338nm;流速:1mL/min,用HPLC法测得穗花杉双黄酮含量为56%。结论:用D-101大孔树脂分离纯化岩柏草总黄酮简单可行,精制效果好。用上述色谱条件可用于穗花杉双黄酮的质量控制。
|
| 关 键 词： | 岩柏草 穗花杉双黄酮 大孔树脂 |
Separation and Purification of Total Flavonoids from Selaginella moellendorfii Hieron by Macroporous Resin and Detected by HPLC |
| |
| FANG Hong-ying,ZHUNG Rang-xiao,WANG Fu-gen,XI Jian-jun,ZHANG Yi-qi,MIAO Liang-bin. Separation and Purification of Total Flavonoids from Selaginella moellendorfii Hieron by Macroporous Resin and Detected by HPLC[J]. Chinese Archives of Traditional Chinese Medicine, 2011, 0(12): 2796-2798 |
| |
| Authors: | FANG Hong-ying ZHUNG Rang-xiao WANG Fu-gen XI Jian-jun ZHANG Yi-qi MIAO Liang-bin |
| |
| Affiliation: | FANG Hong-ying,ZHUNG Rang-xiao,WANG Fu-gen,XI Jian-jun,ZHANG Yi-qi,MIAO Liang-bin(The Sixth Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou 310014,Zhejiang,China) |
| |
| Abstract: | Objective:The study was to investigate the optimal technological parameters of the separation and purification process of Selaginella moellendorfii Hieron by D-101 macroporous resin.Method:UVspectrophotometry was used to determine the content of total flavonoids,and the effect of the work solution concentration,the absorption velocity,the concertration of ethanol on the absorption and desorption of macroporous resin by the losingrate,eluting rate,recovery,purity of total flavonoids were evaluated.Result:The... |
| |
| Keywords: | Selaginella moellendorfii Hieron amentoflavone macroporous resin |
| 本文献已被 CNKI 等数据库收录！ |
|
