促红细胞生成素与一氧化氮合酶抑制剂对肝硬化大鼠高动力循环 …

目的 观察促红细胞生成素或一氧化氮合酶（ＮＯＳ）抑制剂Ｎ－硝基－Ｌ－精酸甲酯（Ｌ＿ＮＡＭＥ）对肝硬化大鼠高循环状态的影响。方法 制造肝硬化大鼠模型,用Ｌ－ＮＡＭＥ胃管内注入或促红素（１００Ｕ／ｋｇ）皮下注射治疗２周;用＾５７Ｃｏ标记微球技术,观察大鼠血流动力学指标;用荧光比色法测血清一氧化氮（ＮＯ）含量。结果 肝硬化大鼠全部出现高动力循环状态,血清ＮＯ含量显著高于正常对照组,促红素和Ｌ－ＮＡＭＥ治

Effects of erythropoietin or nitric oxide synthesis inhibitor on hyperdynamic circulatory state in cirrhotic rats

OBJECTIVE: To observe the effects of erythropoietin or nitric oxide synthesis (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on hyperdynamic circulatory state in rats with cirrhosis. METHODS: Cirrhotic rat model was made. Cirrhotic rats were treated with NOS inhibitor L-NAME (0.5 mg.kg-1.d-1) by gavage or with erythropoietin (100 U/kg) injected subcutaneously for two weeks. The hemodynamic parameters in cirrhotic rats treated with L-NAME or with erythropoietin were determined by using 57Co-labled microsphere technique. Serum nitric oxide (NO) levels were also measured by using a fluorometric assay. RESULTS: Hyperdynamic circulatory state was observed in all rats with cirrhosis. Serum NO levels in cirrhotic rats were significantly higher than that in normal controls. Hyperdynamic circulation status in cirrhotic rats treated with erythropoietin or with L-NAME was markedly attenuated. As compared with untreated-cirrhotic rats, serum NO concentration in erythropoietin-treated and L-NAME-treated cirrhotic rats were significantly lower. CONCLUSION: L-NAME treatment could reverse the hyperdynamic circulatory state in cirrhotic rats which might be ameliorated by inactivation of overproduced NO by increasing hemoglobin with erythropoietin.