Abstract: | Ethanol at concentration of 200 mM induces anesthesia in experimental animals and depresses neurotransmission in isolated spinal cords. To determine whether actions on primary afferent nerve terminals contribute to ethanol's depressant effects on spinal cord, a study was undertaken to test whether ethanol blocks sodium currents (INa) in dorsal root ganglion neurons (DRGn). Whole-cell patch clamp was used to examine INa in DRGn isolated from 1- to 15-day-old rats. At a holding potential of −80 mV ethanol (200 mM) decreased peak tetrodotoxin-resistant (TTX-R) and tetrodotoxin-sensitive (TTX-S) INa by 19.0% ± 2.7 (mean ± SEM) and 8.5% ± 2.2, respectively. Maximal available INa was reduced to 82 ± 4% (TTX-R) and 93 ± 1% (TTX-S) of control. Steady-state inactivation curves were shifted in the hyperpolarizing direction by 2.1 ± 0.2 mV (TTX-R) and 1.1 ± 0.1 mV (TTX-S). At prepulse potentials of −30 mV (TTX-R) and −70 mV (TTX-S), these shifts contributed an additional 17 ± 1% (TTX-R) and 7 ± 1% (TTX-S) reduction in available INa. Ethanol thus selectively induced both voltage-independent and voltage-dependent block of TTX-R INa in DRGn. Because DRGn TTX-R sodium channels are associated with small-diameter primary afferent fibers, these results are consistent with a role for ethanol actions on sodium channels in depression of nociceptive-related neurotransmission in spinal cord. J. Neurosci. Res. 54:433–443, 1998. © 1998 Wiley-Liss, Inc. |